Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

AKT1 APC Antibody

Product Sizes
50 ug
200-326-I51-50UG
About this Product
SKU:
200-326-I51
Additional Names:
AKT1|mouse anti-AKT1 antibody APC conjugation, allophycocyanin conjugated mouse anti-AKT-1 antibody, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma
Application:
Dot Blot, ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
14E5.A2.B2.H9
Clonality:
Monoclonal
Conjugate:
APC
Concentration:
1 mg/ml
Extra Details:
AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT1 (MOUSE) APC conjugated Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
Formulation:
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein followed by monoclonal development.
Isotype:
IgG2a, IgG2a, kappa
Physical State:
Lyophilized
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Anti-AKT1 antibody is directed against human AKT1. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivit
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Plate was coated with monoclonal anti AKT1 antibody (capture antibody) followed by incubation with recombinant AKT1 (p/n 009-001-P21), AKT2 (p/n 009-001-P22), AKT3 (p/n 009-001-P23) proteins. Binding was detected with biotinylated monoclonal anti-AKT pS473. The signal shows specificity of the monoclonal anti-AKT1 antibody to recombinant isoform AKT1 protein and not the isoform 2 and 3.
Flow Cytometry of Mouse anti-AKT1 antibody. Cells: LNCap Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT1 antibody at 1.0 ug/mL for 20 min at 4°C.
Dot Blot of AKT1 Allophycocyanin Conjugated Monoclonal Antibody. Antigen: AKT1 non-phosphorylated. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Primary antibody: none. Secondary antibody: AKT1 Allophycocyanin Conjugated Monoclonal Antibody at 1:1,000 in MB-070 for 60 min at RT. Block: MB-070 for 60 min at RT.