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Antibodies

Glucose Oxidase Antibody

Product Sizes
50 mg
200-1137-50MG
About this Product
SKU:
200-1137
Additional Names:
goat anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody|gox
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
10 mg/ml
Extra Details:
Glucose Oxidase enzyme is widely applied for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Furthermore, glucose oxidase-producing molds such as aspergillus and penicillium species are used for the biological production of gluconic acid.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Glucose Oxidase [Aspergillus niger]
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Yeast/Fungi
Shipping Conditions:
Ambient
Specificity:
Anti-Glucose Oxidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glu
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Rockland Goat anti Glucose Oxidase antibody was used to detect Glucose Oxidase under reducing and non-reducing conditions. Reduced samples of purified Glucose Oxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Rockland Goat anti-Glucose Oxidase antibody (p/n 200-1137, lot 165) at 1:5K in MB-070, ON 4°C. Primary antibody was detected with Rockland Dylight 649 conjugated Donkey anti-Goat (p/n 605-743-125, lot 20834) at 1:10K 1.5 hr RT in MB-070 and imaged on the BioRad VersaDoc imaging system.
Western Blot of Goat anti-Glucose Oxidase antibody. Lane 1: Glucose Oxidase. Load: 50 ng per lane. Primary antibody: Glucose Oxidase antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody (p/n 605-4302) at 1:40,000 for 30 min at RT. Block: (p/n MB-070) for 30 min at RT. Predicted/Observed size: 65-70 kDa, 65-70 kDa for Glucose Oxidase. Other band(s): None.
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