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Antibodies

IL-6 Antibody

Product Sizes
25 ul
109-401-310S-25UL
About this Product
SKU:
109-401-310S
Additional Names:
HSF, Hybridoma growth factor, HGF, Hybridoma plasmacytoma growth factor, IL 6, IL6 protein, IFNB2, Interferon beta 2, BSF2, CDF, B-Cell Differentiation Factor, B-cell stimulatory factor 2, CTL differentiation factor, rabbit anti-Interleukin 6 Antibody, rabbit anti-IL-6|IL6
Application:
Dot Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
80 mg/ml
Extra Details:
Anti-IL-6 Antibody recognizes human IL-6 that is a secreted cytokine with a wide variety of biological functions. IL-6 is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Anti-IL6 antibody is ideal for investigators involved in Immunology and Cancer research.
Format:
Antiserum
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-IL-6 antiserum detects recombinant and native human IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). This product has minimal reactivity with mouse IL-6.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western blot using Rockland's Anti-IL6 antibody. Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated Goat anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
Western Blot of Rabbit Anti-IL-6 Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Human Rec. IL-6/HeLa Whole Cell Lysate [0.05ug/10ug]. Lane 3: Human Rec. IL-6/HeLa Whole Cell Lysate [0.02ug/10ug]. Lane 4: Human Rec. IL-6/HeLa Whole Cell Lysate [0.01ug/10ug]. Lane 5: HeLa Whole Cell Lysate (p/n W09-000-364) [10ug]. Primary Antibody: Anti-IL-6 at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP conjugate (p/n 611-103-122) at 1:70,000 for 30mins at RT. Block: BlockOut Buffer (p/n MB-073). Predicted MW: ~23kDa. Observed MW: ~19 Exposure: 2 seconds.