Mouse Anti-FLAG Antibody
Product Sizes
0.5 mg
OAPA00047-0.5MG
About this Product
- SKU:
- OAPA00047
- Buffer:
- Lyophilized PBSPhysical Appearance: Sterile filtered white freeze-dried powder
- translate.label.attr.clone:
- NYRFLAG
- Clonality:
- Monoclonal
- Extra Details:
- A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody. denoted as M1. binds the peptide in the presence of bivalent metal cations. preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide. The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins.
- Formulation:
- Lyophilized PBSPhysical Appearance: Sterile filtered white freeze-dried powder
- Host:
- Mouse
- Immunogen:
- Flag-conjugated proteins.
- Isotype:
- IgG1, kappa
- Physical State:
- Lyophilized
- Purification:
- Protein A Purified; Ion Exchange
- Shipping Conditions:
- Ambient
- Specificity:
- Will recognize FLAG (DYKDDDDK) conjugated at either the amino or carboxy terminus. Can be used bound to various resins for affinity purifications.
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Aviva Systems Biology
- Type:
- Antibody: Monoclonal Antibody
- Manufacturer's Data Sheet:html_datasheet.php
