ZytoPure® FISH Probes for Lung Cancer

Zytomed Systems is proud to offer the new product line ZytoPure^® FISH, a reliable and accurate tool for the detection of genetic aberrations in FFPE tissue sections, cell samples and metaphase spreads by fluorescence in situ hybridization. ZytoPure^® FISH Probes display brilliant signals, low background, and an excellent signal to noise ratio. They are intended to be used in combination with the ZytoPure^® FISH Accessory Kit comprising all reagents necessary for performing a FISH procedure on FFPE sections. The kit contains just 4 reagents and includes a robust and easy to follow protocol. All ZytoPure^® FISH Probes as well as the ZytoPure^® FISH Accessory Kit are CE/IVD-labelled.

Background information

Rearrangements of receptor tyrosine kinases (RTK) ALK, ROS1, and RET determine clinically important molecular subgroups of non-small cell lung cancer (NSCLC). About 3 % – 5 % of all NSCLCs show an ALK rearrangement, and 1 % - 2 % show ROS1 or RET rearrangements [1]. In all fusion variants, the 3´ TK domain is fused to the promotor regions and 5´ coding regions of the fusion partner. Hence, rearranged RTKs are permanently active, sending proliferation signals into the cell, and thereby promoting non-controlled cell growth.

Several very efficient TK inhibitors (TKIs) were launched in recent years [2], and as a consequence the median survival of an ALK positive NSCLC patient treated with two ALK inhibitors is now more than 5 years [3]! Amplification of the MET gene is the second most common resistance mechanism of lung adenocarcinomas against EGFR TKIs [4]. In addition, primary MET amplification is also found in a small percentage of treatment-naïve lung adenocarcinomas [5].

Product information

ZytoPure^® FISH Probes for lung cancer

Ancillary Reagents