SoluLINK® Bioconjugation
The SoluLINK Bioconjugation products offer unique features to help researchers and manufacturers label reagent consistently and measurably in 4-6 hours, as the HyNic-4FB conjugation reaction can be tracked spectrophotometrically. This ability to control the reaction avoids wastage of precious proteins caused to precipitate out of solution if they are over conjugated, and GMP standards can be achieved.
With a range of supporting reagents, SoluLINK can assist with the bioconjugation required for a range of assays, such as ELISAs, affinity chromatography, flow cytometry, and bead assays. Gentle chemistries and stable bond formation – to 92°C and pH 2.0 - 10.0, means the SoluLINK reagent is a conjugation method of choice.
SoluLINK bioconjugation technology is engineered for high-performance conjugation of all classes of biomolecules, including antibodies, proteins, peptides, oligonucleotides, carbohydrates, drugs and surfaces. The result is reliable, quantifiable labelling delivering ready-to-use conjugates at high yield.
| Conjugate | Use |
|---|---|
| Oligonucleotide-antibody conjugates | Proximity Ligation Assays (PLA) |
| Oligonucleotide-KLH conjugates | Anti-Oligonucleotide antibody synthesis |
| Carbohydrates-KLH conjugates | Production of anti-carbohydrates antibodies |
| Bacterial carbohydrate-bead conjugates | Diagnostic assays |
| siRNA-antibody conjugates with disulfide cleavible linker | siRNA delivery |
| siRNA-cationic peptide conjugates | siRNA delivery |
| Drug-protein conjugates | Drug delivery |
| Drug-KLH conjugates | Immunodetection |
Each step of the conjugation can be controlled to ensure ideal labelling of each biomolecule, and the final conjugation can be tracked using a spectrophotometer.
Conjugates can be stable for 6 months once prepared & purified and 4FB labelled reagents are often stable for many weeks. (HyNic labelling should be done just prior to conjugation to avoid dimer formation)
<= Solulink's easy to use, linker-based technology.
Ancillary Reagents
To give you ultimate flexibility, many of the SoluLINK reagents are available as separate reagents, enabling you to build your own conjugation.
| A Type of conjugate | B Type of molecule | C Reactive Group | D SoluLINK Product | |
|---|---|---|---|---|
| Antibody-Protein | Ab | If using an Amino (NH2) then use: | Linker 1 | S-HyNic (S-1002-105) |
| If using a Thiol (SH2) then use: | MHPH (S-1009-010) | |||
| Protein | If using an Amino (NH2) then use: | Linker 2 | S-4FB (S-1004-105) | |
| Protein -Oligos | Protein | If using an Amino (NH2) then use: | Linker 1 | S-HyNic (S-1002-105) |
| If using a Thiol (SH2) then use: | MHPH (S-1009-010) | |||
| Oligo | If using a 3’ or 5’ Amino, then use: | Linker 2 | S-4FB (S-1004-105) | |
| Protein -MagnaLINK Beads conjugation | MagnaLINK Beads | If using 4FB Magnetic beads | Linker 1 | MagnaLINK 4FB Magnetic Beads (M-1004-010) |
| AB, other protein, R-PE, APC, perCP, HRP, AlkPhos | If using an Amino (NH2) then use: | Linker 2 | S-HyNic (S-1002-105) | |
| If using a Thiol (SH2) then use: | MHPH (S-1009-010) |
Instructions: SoluLINK technology requires 2 linkers to successfully conjugate 2 biomolecules to give you a quantifiable, controllable and stable result.
Step 1: Select type of conjugate (A)
Step 2: Select the 1st biomolecule (B) then select its reactive group (C). The SoluLINK product you require is in column D (Linker 1)
Step 3: Select the 2nd biomolecule (B) then select its reactive group (C). The SoluLINK product you require is in column D (Linker 2)
Step 4: Order the products referenced in steps 2 & 3 for a successful conjugation.
ChromaLINK™ Reagents
ChromaLINK offers a quick way to label proteins with biotin or digoxigenin, taking approximately 2.5 hours labelling time, and 5 minutes to quantify – thanks to the incorporated bis-aryl hydrazone chromophore that measures labelling using just a spectrophotometer. There is a range of options depending on the solubility requirements of your protein, and the quantities of protein to be labelled.
| Product | Size | SKU |
|---|---|---|
| Biotin | ||
| ChromaLINKTM Biotin Labelling Kit | Kit - labels 25ug to 1mg | B-9007-105K |
| ChromaLINKTM One-Shot Antibody Biotinylation Kit | Kit - labels 100ug of Ab | B-9007-009K |
| ChromaLINKTM One-Shot Antibody Biotinylation Kit (Large Scale) | Large-Scale - labels 2mg of Ab | B-9007-020K |
| ChromaLINKTM One-Shot Antibody Biotinylation Kit (Custom Size) | Labels any amount | Please Inquire |
| ChromaLINKTM Biotin Labelling Reagent (Sulfo Version Water Soluble) | 10mg | B-1007-110 |
| ChromaLINKTM Biotin Labelling Reagent (Sulfo Version Water Soluble) | 5 x 1.0mg | B-1007-105 |
| ChromaLINKTM Biotin Labelling Reagent (DMF Soluble) | 10mg | B-1001-010 |
| ChromaLINKTM Biotin Labelling Reagent (DMF Soluble) | 5 x 1.0mg | B-1001-105 |
| ChromaLINKTM Biotin Malemide Reagent | 10mg | B-1012-010 |
| Digoxigenin | ||
| ChromaLINKTM Digoxigenin One-Shot Antibody Labelling Kit | Kit - labels 100ug of Ab | B-9014-009K |
| ChromaLINKTM Digoxigenin One-Shot Antibody Labelling Kit | Large-Scale - labels 2mg | B-9014-020K |
Complete Kits
Kits containing all you need to simply label and purify your protein using SoluLINK or ChromaLINK technology.
| Product | Size | SKU |
|---|---|---|
| HRP- Antibody All-In-One Conjugation Kit | Kit- conjugates 2x 100µg Ab | A-9002-001 |
| R-PE- Antibody Conjugation Kit | Kit- conjugates 2x 0.5mg Ab | P-9002-002 |
| Protein-Protein Conjugation Kit | Kit- labels 2x 650µg of protein (>7kDa) | S-9010-1 |
| Protein -Oligo Conjugation Kit | Kit- labels 2x 650µg of protein to Oligo (20-120 nucleotides) | S-9011-1 |
| Fluorescein One-Shot Antibody Labelling Kit | Kit- labels 2x 100µg of Ab | F-9001-009K |
| ChromaLINK ™ Biotin Labelling Kit | Kit -Labels 25µg to 1mg | B-9007-105K |
| ChromaLINK™ One-Shot Antibody Biotinylation Kit | Kit – Labels 100µg of Ab | B-9007-009K |
| ChromaLINK ™ Digoxigenin One-Shot Antibody Labelling Kit | Kit - Labels 100µg of Ab | B-9014-009K |
Magnetic beads
The SoluLINK NanoLINK beads have been engineered with a unique streptavidin crosslinking to allow the highest biotin binding capacity for beads of their size – up to 12x higher than other brands.
Designed for use in applications such as antibody based cell separation, IVD Immunoassay development, ChIP and DNA/RNA binding protein isolation, immunoprecipitation and protein isolation and next gen sequencing target enrichment.
| Molecule | NanoLINK (0.8um) binding capacity | MagnaLINK (2.8um) binding capacity |
|---|---|---|
| Free Biotin | >14 nmol/mg | >12 nmol/mg |
| Biotinylated oligo (23-mer) | >2.5 nmol/mg | >0.8 nmol/mg |
| Biotinylated IgG (3 biotins per IgG) | >250 ug/mg | >112.6 ug/mg |
The MagnaLINK beads offer exceptional uniformity in size, and prepared ready for covalent immobilization for user defined, high performance affinity purification schemes.
The 4FB MagnaLINK magnetic beads offer an alternative to biotin binding, using the SoluLINK chemistry – this offers an efficient way to conjugate any HyNic labelled proteins and antibodies to beads, when compared to other existing methods.