UltraMarathonRT Two-Step RT-qPCR Kit
Unparalleled performance, improving and simplifying gene expression analysis with RNAConnect!
The UltraMarathonRT® Two-Step RT-qPCR Kit sets a new standard in RNA quantification, delivering unmatched sensitivity, reliability, and robustness for researchers. Most RT enzymes used in RT-qPCR have low processivity and are only able to cover relatively unstructured regions, which restricts qPCR primer design to limited regions of the transcript. Importantly, even when sophisticated software is used for primer design, different primer pairs can result in dramatically different C values for the same target when using on-processive RTs. This variability introduces uncertainty as to whether true gene expression levels have been identified by RT-qPCR or not. The new UltraMarathonRT RT-qPCR Kit solves this problem.
UltraMarathonRT provides Reliable Quantification Regardless of qPCR Primer Location
With intrinsic helicase activity, UltraMarathonRT (uMRT) swiftly navigates high GC content and RNA secondary structures, providing full-length cDNA synthesis. Having full-length cDNA as a template for the qPCR step enables easy primer pair selection along any section of the transcript without having to worry about sequence or structure-associated hurdles.
A) Diagram of the relative locations of the 10 primer pairs used for qPCR. B) Distribution of GC% for a30-base window across the SRGAP2C gene. The GC% ranges from very high (>75%) to very low (<10%). C) The C values for four different RT-qPCR kits across all 10 primer pairs. Because the uMRT kit shows no 5’ to 3’ biasand no bias for GC content, the results of the qPCR are very consistent. The competing kits have highly variableresults.

UltraMarathonRT Has a Broad Dynamic Range Delivering Accurate Quantification of All RNAs
When identifying and quantifying gene expression differences, it is essential to be able to compare transcripts, whether highly expressed or rare. This kit excelsin detecting a wide range of RNA types, including low abundance, viral, non-coding, long, and structured RNAs. Its sensitivity and efficiency make it indispensable for complex RNA studies, accommodating RNA input concentrations from an 8-log range (0.1 pg to 2 μg). With such a broad dynamic range and high efficiency, the kit supports diverse experimental needs with ease.
The UltraMarathonRT Two-Step RT-qPCR Kit accurately detects RNA inputs from 0.1 pg to > 100 ng with high efficiency and reliability.
UltraMarathonRT Motors Past Troublesome cDNA Synthesis Inhibitors
The ultra-processive UltraMarathonRT enzyme has been engineered to synthesise full-length cDNA from the most difficult, long, highly structured RNA templates, even in the presence of potential enzyme inhibitors. The uMRT RT-qPCR Kit delivers robust, consistent results when faced with a group of inhibitors commonly found in biological samples, including ethanol, isopropanol, TRIzol, formalin, ammonium acetate, and hematin.
Heat Destroys Your RNA Samples
In the past, the only way to unwind RNA secondary structures so that MMLV RTs can get through them was to heat the delicate RNA samples. Each successive generation of MMLV RTs sought higher levels of thermostability to accommodate the excessive temperatures necessary to melt these structures. However, heat rapidly degrades the samples you’re interested in. UltraMarathonRT’s intrinsic helicase activity unwinds all such secondary structures at ambient temperatures, preserving the integrity of your RNA samples.
Perform the Two-Step in Less Than an Hour
The UltraMarathonRT Two-Step RT-qPCR Kit ensures unmatched data reliability, delivering consistent C values regardless of RT primer type, PCR primer location or RNA complexity. Whether working with gene-specific primers, oligo(dT), or random primers, researchers can trust this kit to provide reproducible results. Its robust design eliminates biases caused by variable GC content, stable RNA structures and repeat sequences, ensuring accurate quantification across all templates. Inhibitory compounds often complicate workflows, but the UltraMarathonRT kit is engineered for high inhibitor tolerance. It maintains exceptional performance in the presence of common inhibitors such as ethanol, isopropanol, TRIzol, formalin, and more. Additionally, its fast reaction and streamlined protocol allows researchers to generate results in under an hour, while dye-based detection facilitates real-time RNA monitoring. The kit’s applications extend beyond standard gene expression analysis.
It is ideal for RNA-Seq verification, quantifying small RNAs like miRNAs, analysing alternative splicing, and assessing RNA degradation or editing. Whether verifying RNAi knockdowns or evaluating CRISPR experiments, this kit provides reliable and precise results, making it a trusted companion for modern RNA studies.