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CARD Hyper-Ova

CARD HyperOva® (Superovulation Reagent for mouse) is an ultra-superovulation reagent that is 3-4 times more efficient at inducing ovulation than the conventional PMSG-hCG method (80 ova per C57BL/6 mouse versus approximately 20). CARD HyperOva® is also effective with many other strains. Even further gains in efficiency can be realized by using CARD MEDIUM® during the IVF procedure.

 

 

  1. Inject 0.1-0.2 mL CARD HyperOva® i.p. into a 26-30 days old female mouse (birthdate = 0). Injections are usually performed during the light cycle, between 17:00 and 18:00.
  2. At 48 hours after CARD HyperOva® recipients are injected i.p. with 7.5 IU human chorionic gonadotropin (hCG) (not included).

Why Use CARD HyperOva®?

  • Reduce Animal Use (contribute to the 3Rs of animal welfare: Replacement, Reduction, Refinement)
  • Using CARD HyperOva® can reduce the number of oocyte donor mice by a factor of 1/3-1/4.
  • Optimize creation and preservation of genetically modified mice.
  • More ova from fewer mice using CARD HyperOva® facilitates in vitro fertilization and embryo transfer.
  • More ova from fewer mice using CARD HyperOva® enables the large-scale preparation of embryos.
  • Get more done!