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OxiSelect HNE Adduct Competitive ELISA Kit

2BScientific Limited presents one of the most sought-after products from Cell Bio Labs the OxiSelect HNE Adduct Competitive ELISA Kit that is a powerful, highly sensitive and easy to use tool for assessing Oxidative Stress Research. 

Lipid peroxidation is a well-defined mechanism of cellular damage in animals and plants. This unstable indicator of oxidative stress forms more complex and reactive compounds such as Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), a natural bi products of lipid peroxidation.  Both MDA or HNE can cause structural and functional changes to the protein, specifically 4-HNE that can react with lysine, histidine, or cysteine residues in protein to form adducts causing cellular damage and changes in cell behaviour.  Detecting 4-HNE is important for several reasons:

  • 4-HNE serves as a stable and specific marker of lipid peroxidation, providing insights into the extent of oxidative damage in cells and tissues.
  • 4-HNE can modify proteins, DNA, and other biomolecules, affecting their function and contributing to disease progression. Monitoring 4-HNE levels helps in understanding its role in various pathological conditions and effects on cellular metabolic pathways. 
  • Measuring 4-HNE can be used to evaluate the efficacy of antioxidants and other therapeutic agents aimed at reducing oxidative stress and its associated damage.

 

Key benefits of the OxiSelect HNE Adduct Competitive ELISA Kit

 

  • Specificity and Sensitivity: This assay is designed to specifically detect and quantify HNE-protein adducts, which are stable markers of oxidative stress. The high sensitivity allows for the detection of low levels of HNE adducts in various sample types
  • Versatility: The kit can be used with a wide range of samples, including cell and tissue lysates, serum, plasma, and purified proteins from animals and plants . This versatility makes it suitable for diverse research applications
  • Quantitative Results: The competitive ELISA format provides quantitative results by comparing the absorbance of unknown samples to a standard curve. This allows for precise measurement of HNE adduct levels
  • Ease of Use: The assay is straightforward to perform, with a clear protocol and all necessary reagents included in the kit. This makes it accessible even for researchers with limited experience in ELISA techniques

 

This highly effective mechanism can make your Oxidative stress Research easier by helping to gain greater results on the status of the cells and understand effectiveness of interventions designed to counteract oxidative damage.