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Proteins and Peptides

Vector[R] TrueVIEW[R] Autofluorescence Quenching Kit

Product Sizes
1 kit
SP-8400-1KIT
About this Product
SKU:
SP-8400
Additional Names:
Blocking Reagents [Blocking Reagents ]
Application:
Immunofluorescence
Conjugate:
Quencher
Extra Details:
Features: Removes unwanted autofluorescence in tissue sections due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin Improves overall signal-to-noise in most immunofluorescence assays Effective on problematic tissues such as kidney and spleen Easy-to-use, one-step method Compatible with a wide selection of fluorophores Compatible with standard epifluorescence and confocal laser microscopes
Shipping Conditions:
Ambient
Storage Conditions:
2-8[o]C
Supplier:
Vector Laboratories
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Fluorophores
Why is the TrueVIEW™ Autofluorescence Quenching Reagent applied after completion of the IF assay and not at the start of the procedure?:
During product development, applying the TrueVIEW™ Quenching reagent at the end of our standard IF procedure yielded the most optimal reduction in autofluorescence signal. The reagent is retained on the tissue at the time of mounting, allowing for extended quenching action, with little to no effect on the specific fluorescent signal. However, in applications with very brief staining procedures, such as a primary antibody directly conjugated with a fluorophore, application of TrueVIEW™ Quenching reagent may be just as effective at the start of the procedure.
Is TrueVIEW effective against lipofuscin derived autofluorescence?:
The working solution of TrueVIEW works in an electrostatic manner to greatly reduce or eliminate fluorescence in tissue sections induced through the use of an aldehyde-based fixative. TrueVIEW is also effective at reducing fluorescence from tissue components such as collagen, elastin and red blood cells. TureVIEW does not work to reduce autofluorescence due to lipofuscin.
How long can I store the TrueVIEW working solution?:
Once made up, the working solution of TrueVIEW can be stored either on the bench top or in the fridge (2-8C) for about 48 hours (2 days) without loss of activity or function. Following this time we would suggest discarding unused working solution and make fresh solution as required.
My tissue sections turned blue when I applied TrueVIEW. Is this supposed to happen?:
Yes, the working solution is a blue colour that does "stain" the tissue section blue. This indicates an active and appropriate chemical reaction is occurring. The blue stain on the section does not fluoresce and does not interfere with the immunofluorescence application.
What mounting media can I use with TrueVIEW?:
Both TrueVIEW products, SP-8400 and SP-8500, are supplied with 2 mL of VECTASHIELD Vibrance antifade mounting media. We have found that the mounting media does play a crucial role in maintaining the high signal to noise ratio when using TrueVIEW. VECTASHIELD Vibrance is included therefore as a critical component of the kit, and it is recommended to use the media supplied for optimal results. At this time, we do not have sufficient data to confidently recommend the use of other vendors mounting media with TrueVIEW. Substitution of VECTASHIELD Vibrance with another mounting media may result in less than satisfactory results.