Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Histone H3 K27me2 Antibody

Product Sizes
50 ug
600-401-V34-50UG
About this Product
SKU:
600-401-V34
Additional Names:
HIST1H3A|Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/e, Histone H3/f, Histone H3/g, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Application:
ChIP, Dot Blot, ELISA, Immunofluorescence, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
2.63 mg/ml
Extra Details:
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Levels of H3K27 dimethylation are higher in silent genes than in active genes suggesting that this histone modification is associated with transcriptional repression. Anti-H3K27me2 Antibody is ideal for research in Chromatin Remodeling, Epigenetics and Gene Expression.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-H3K27me2 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide of human histone H3, dimethylated at lysine 27.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Fish, Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-H3K27me2 Antibody was purified by affinity purification. This product reacts with human Histone 3 dimethyl Lys27. Cross reactivity with H3K27me2 from species other than human is not known.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence Microscopy results of Rabbit anti-Histone H3 K27 me2 antibody. Tissue: HeLa cells. Fixation: 4% formaldehyde for 10 min. Block: PBS/Triton X-100 / 5% normal goat serum and 1% BSA. Primary antibody: Histone H3 K27 me2 antibody at 1:500 for 1 hr at RT. Secondary antibody: anti-rabbit Alexa 488 secondary antibody at 1:10,000 for 45 min at RT. Staining: Histone H3 K27 me2 antibody as green fluorescent signal (left), with DAPI (middle), and a merge of the two stainings (right).
Western Blot results of Rabbit anti-Histone H3 K27 me2 antibody. Lane 1: 15 ug of histone extracts from HeLa cells. Primary antibody: Rabbit anti-Histone H3 K27 me2 antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase anti-rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: TBS-Tween / 5% BLOTTO. Predicted/Observed size: ~15 kDa for Rabbit Histone H3 K27 me2.
Dot Blot results of Rabbit anti-Histone H3 K27 me2 antibody. Antigens: H3K27, H3K27me1, H3K27me2, H3K27me3, H3K27me3 S28p, H3K4me2, H3K9me2, H3K36me2, H3K79me2, H4K20me2. Load: 100pmol, 50pmol, 25pmol, 5pmol, 2pmol, 0.2pmol, and control. Primary antibody: Histone H3 K27 me2 antibody at 1:50,000 for 45 min at 4°C. Secondary antibody: anti-rabbit HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO.
ELISA results of Rabbit anti-Histone H3 K27 me2 antibody. Antigen: Histone H3 K27 me2. Coating amount: 0.1 ug per well. Dilution series: serial. Titer: 1:480,000 Histone H3 K27 me2 antibody. Substrate: TMB (p/n TMBE-1000).
Chromatin Immunoprecipitation Rabbit H3K27me2 Antibody. ChIP assays were performed using HeLa cells, the Anti-H3K27me2 Antibody, and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analyzed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the promoter of the inactive HBB and the coding region of the inactive MYOD1 genes, used as positive controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me2 is preferably present at silent genes.