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Antibodies

CH25H Antibody

Product Sizes
100 ug
600-401-MM8-100UG
About this Product
SKU:
600-401-MM8
Additional Names:
CH25H|Rabbit Anti-Cholesterol 25-Hydroxylase, Rabbit Anti-CH25H, Cholesterol 25-Monooxygenase, EC 1.14.99.38, H25OH, C25H
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
0.92 mg/ml
Extra Details:
CH25H (Cholesterol 25-Hydroxylase catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. CH25H plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for several lymphoid cells. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Anti-CH25H Antibody is useful for researchers interested in Alzheimer's Disease, Neuroscience, and metabolism.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-CH25H antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of human CH25H conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against human CH25H. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence and 92% homology to pig, 91% homology to rat, and 75% homology to mouse.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry of Rabbit Anti-CH25H Antibody. Tissue: Human Kidney. Fixative: None. Antigen Retrieval: HIER using Citrate Buffer for 20 minutes. Primary Antibody: Anti-CH25H at 1:100 for 30 min at RT. Secondary Antibody: Anti-Rabbit Poly-HRP IgG Ready-to-Use for 8 minutes at RT. Counterstain: Hematoxylin. Substrate: DAB. Staining: (A) Negative control. (B) 20X. (C) 40X. Results: Shows strong cytoplasmic staining of renal tubular epithelium.
Western Blot of Rabbit Anti-CH25H Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: CH25H overexpressed HEK293T Lysate [10ug]. Lane 3: HeLa whole cell Lysate (p/n W09-000-364) [10ug]. Primary Antibody: Anti-CH25H at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 for 30min at RT. Block: Universal BlockOut Buffer (p/n MB-073). Predicted MW: ~31.7kDa. Observed MW: ~35kDa. Exposure: 3 seconds.