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Antibodies

Ankrd26 Antibody

Product Sizes
100 ug
600-401-917-100UG
About this Product
SKU:
600-401-917
Additional Names:
Ankrd26|rabbit anti-Ankrd26 Antibody, ankrd 26, ankrd-26, Ankyrin repeat domain 26 antibody, Ankyrin repeat domain containing protein 26 antibody, bA145E8.1 antibody, KIAA1074 antibody
Application:
ELISA, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.48 mg/ml
Extra Details:
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. POTE gene is expressed in prostate and breast cancer and a potential candidate for immuno-therapy. It is also expressed in undifferentiated embryonic stem (ES) cells. ANKRD-26 is the ancestral gene of POTE and has a homologue in mouse. Mouse Ankrd26 is also selectively expressed in ES cells and various cancers but not in other adult tissues. The encoded protein of Ankrd26 contains ankyrin repeats and coiled coil domain of spectrin suggestive of a signaling molecule.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near amino acids 1-25 of mouse Ankrd26 protein.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse
Shipping Conditions:
Dry Ice
Specificity:
This affinity-purified antibody is directed against mouse Ankrd26 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Ankrd26 proteins from mouse (100%), rat (93%), bovine (81%) and human (68%). Reactivity against homologues from other sources is not known.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western blot using Rockland's affinity purified anti-Ankrd26 antibody shows detection of a band at ~81 kDa corresponding to mouse Ankrd26 protein. Lane 1 Blank, Lane 2 MES cell lysate - 80 ug, Lane 3 MES cell lysate - 40 ug, Lane 4 293T-ANKRD26 transfected cell lysate - 20 ug, Lane 5 control 293T cell lysate - 20 ug, Lane 6 BSA-ANKRD26 conjugate 20 ng, Lane 7 BSA - 500 ng, Lane 8 BSA - 100 ng, Lane 9 BSA 20 ng and Lane 10 Protein standards. Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000 followed by detection using ALP conjugated Gt-a-Rabbit IgG (611-105-122 is suggested) diluted to 1:3,000. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication. Ira Pastan, NIH, CCR, Bethesda, MD.