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Antibodies

Eaat2 Antibody

Product Sizes
100 ug
600-101-MN3-100UG
About this Product
SKU:
600-101-MN3
Additional Names:
Goat Anti-Eaat2 Antibody, Goat Anti-Excitotoxic Amino Acid Transporter Antibody, Solute Carrier Family 1 Member 2, Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter) Member 2, Sodium-Dependent Glutamate/Aspartate Transporter 2, Toxcitatory Amino Acid Transporter, Glutamate/Aspartate Transporter II, EAAT2, Excitotoxic Amino Acid Transporter, EIEE41, GLT-1, HBGT, GLT1|Slc1a2
Application:
ELISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.05 mg/ml
Extra Details:
SLC1A2 (Solute Carrier Family 1 Member 2), glutamate transporters, also known as excitatory amino acid transporters (EAATs), are sodium- and potassium- dependent members of the solute carrier family 6 (SLC1), widely distributed throughout the brain. These sodium-dependent, high-affinity amino acid transporter mediates the uptake of L-glutamate, L-aspartate, and D-aspartate functioning as a symporter that transports one amino acid molecule together with two or three Na (+) ions and one proton, in parallel with the counter-transport of one K (+) ion. It mediates the Cl (-) flux that is not coupled to amino acid transport avoiding the accumulation of negative charges due to aspartate and Na (+) symport. Eaat2 is essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate. Improper regulation of this gene is thought to be associated with several neurological disorders including Epileptic Encephalopathy, Early Infantile, 41 and Undetermined Early-Onset Epileptic Encephalopathy. Anti-Eaat2 Antibody is useful for researchers interested in transmembrane transporter activity, neuroscience research, and cell metabolism.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Anti-Eaat2 Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to the N-terminal region of Rat Eaat2 surrounding aa1-25 conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against rat Eaat2. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human, mouse, and rat.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence of Goat Anti-Eaat2 Antibody. Cells: Rat Pups Brain heterogenous cells. Fixation: 4% PFA. Permeabilization: 0.3%Triton X-100. Primary Antibody: Anti-Eaat2 at 15ug/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG DyLight™488 (p/n 605-741-125) at 5ug/mL for 60mins at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI, (B) anti-Eaat2 and secondary, (C) merge A+B, (D) secondary only. Expected localization: plasma membrane >> extracellular > mitochondria, nucleus.
Western Blot of Goat anti-Eaat2 Antibody. Lane 1: Rat Brain Lysate (p/n W12-000-T077) (35ug) [+]. Lane 2: HEK293T whole cell lysate (35ug) [-]. Primary Antibody: anti-Eaat2 at 5ug/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG HRP (p/n 705-703-125) at 1:40,000 for 60mins at RT. Block: 5% BLOTTO (p/n B501-0500). Predicted MW: 62kDa. Observed MW: ~65, 130 kDa. Dimerization expected, with glycosylations and lipidations.
Western Blot of Goat anti-Eaat2 Antibody. Lane 1: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Rat Brain Lysate (p/n W12-000-T077)(35ug) [+]. Lane 3: Rat Liver Lysate (p/n W12-000-T093)(35ug) [-]. Lane 4: Mouse Brain Lysate (p/n W10-000-T004)(35ug) [+]. Lane 5: Mouse Liver Lysate (p/n W10-000-T020)(35ug) [-]. Lane 6: NIH/3T3 Whole Cell Lysate (p/n W10-003-371). Load: 35ug/lane. Primary Antibody: anti-Eaat2 at 1:1000 overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG HRP (p/n 705-703-125) at 1:40,000 for 60mins at RT. Block: BlockOut Buffer (p/n MB-073). Predicted MW: 62kDa. Observed MW: ~65, 130 kDa. Dimerization expected, with glycosylations and lipidations.