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Antibodies

BMAL1 Antibody

Product Sizes
100 ug
600-101-ML8-100UG
About this Product
SKU:
600-101-ML8
Additional Names:
ARNTL|Goat Anti-Aryl hydrocarbon receptor nuclear translocator-like protein 1 Antibody, Goat Anti-Bmal1 Antibody, Arnt3, Brain and muscle ARNT-like 1
Application:
ELISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
0.92 mg/ml
Extra Details:
Arntl (Aryl Hydrocarbon Receptor Nuclear Translocator Like), also known as Bmal1, is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, Angelman Syndrome and altered circadian rhythm. Anti-Bmal1 Antibody is useful for researchers interested in Neuroscience research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Anti-BMAL1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of human Bmal1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against human Bmal1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse, rat, golden hamster, horse, gallus gallus, Pongo abelii, Tyto alba, and Nannospalax galili.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence of Goat anti-BMAL1 Antibody. Cell Line: HeLa Cells. Fixative: 4%PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-BMAL1 at 15ug/mL overnight at 2-8°C. Secondary antibody: Donkey Anti-Goat IgG DyLight™488 (p/n 605-741-125) at 15ug/mL at RT for 1 hr. Nuclear Counterstain: DAPI. Image Staining: (A) DAPI. (B) Anit-BMAL1 + secondary. (C) merge A+B. (D) secondary only.
Western Blot of Goat Anti-BMAL1 Antibody. Lane 1: Opal PreStained Molecular Weight Marker (p/n MB-210-0500). Lane 2: BMAL1/ARNTL HEK293T OverExpressing Lysate c-myc/DDK tag* [+]. Lane 3: HEK293T empty vector lysate [+]. Lane 4: NIH3T3 Lysate (p/n W10-000-358). Lane 5: Mouse Brain Lysate (p/n W10-000-T004). Primary Antibody: Anti-BMAL1 at 1:1000 overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG HRP (p/n 605-703-125) at 1:40,000 for 30 min at RT. Buffer: 5% BLOTTO (p/n B501-0500). Predicted/observed: overexpressing 76kDa, endogenous ~67-69kDa.