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Antibodies

Eaat1 Antibody

Product Sizes
25 ul
200-901-ML1S-25UL
About this Product
SKU:
200-901-ML1S
Additional Names:
Chicken Anti-Slc1a3 Antibody, Chicken Anti-Eaat1 Antibody, Excitatory amino acid transporter 1, Glial glutamate transporter, Sodium-dependent glutamate/aspartate transporter 1, GLAST, GAST-1, Slc1a3, Eaat1|Slc1a3
Application:
ELISA, Immunofluorescence, Western Blot
Buffer:
0.002 M Sodium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
0.99 mg/ml
Extra Details:
Slc1a3 (or Eaat1) is widely distributed throughout the brain. It is a potassium and sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate, L-aspartate, and D-aspartate. It functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion. It mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Slc1a3 plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate. Slc1a3 can play a role in Episodic Ataxia, Type 6 and Wernicke Encephalopathy. Anti-Slc1a3 Antibody is useful for researchers interested in SIDS, Glucose Metabolism, and Glutamate and Amino Acid Binding.
Formulation:
0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0
Host:
Chicken/Avian
Immunogen:
Anti-Slc1a3 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a N-Terminal portion of rat Slc1a3 conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgY
Physical State:
Liquid
Reactivities:
Rat
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against rat Slc1a3. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Blast analysis the immunogen sequence shows 100% identity with mouse and 91.7% human.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence of Chicken Anti-Eaat1 Antibody. Cells: Post-natal rat pup (PND1) heterogeneous brain cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Eaat1 Antibody at 15ug/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Chicken IgG DyLight™488 Conjugated (p/n 603-141-126) at 5ug/mL for 1hr at RT. Nuclear Counterstain: DAPI. Localization expected: Cell membrane (mitochondria, nucleus). Observed staining: mitochondrial. Image Staining: A). DAPI. B). Anti-Eaat1 + secondary. C). Merged A + B. D). Secondary Only.
Western Blot of Chicken Anti-Eaat1 Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: HEK293T lysate [20ug] (p/n W09-001-GX5). Lane 3: Mouse Liver, adult, Whole Cell Lysate [20ug] (p/n W10-000-T020). Lane 4: Rat pup PND2-6 - minimal cortex brain lysate (20ug). Lane 5: Rat pup PND2-6 - minimal cortex brain lysate (40ug). Primary Antibody: Anti-Eaat1 Antibody at 1:500 for overnight at 2-8°C. Secondary Antibody: Goat Anti-Chicken IgG HRP conjugated (p/n 603-103-126) at 1:40,000 for 1hr at RT. Blocking: BlockOut buffer (p/n MB-073). Expected MW: ~54, 60 kDa (2 isoforms). Exposure: 60 seconds.
Immunofluorescence of Chicken Anti-Eaat1 Antibody. Cells: Post-natal rat pup (PND1) heterogeneous brain cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Eaat1 Antibody at 15ug/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Chicken IgG DyLight™488 Conjugated (p/n 603-141-126) at 5ug/mL for 1hr at RT. Nuclear Counterstain: DAPI. Localization expected: Cell membrane (mitochondria, nucleus). Observed staining: punctate staining indicates mitochondrial detection.