DYKDDDDK Tag (Anti-FLAG[R]) Antibody Dylight[TM] 680 Conjugated
Product Sizes
100 ug
200-344-383-100UG
About this Product
- SKU:
- 200-344-383
- Additional Names:
- mouse anti-FLAG™ tag DyLight™680 conjugation, Enterokinase Cleavage Site (ECS), DyLight™680 conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
- Application:
- ELISA, FLISA, Flow Cytometry, Immunofluorescence, Western Blot
- Buffer:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
- CE/IVD:
- RUO
- translate.label.attr.clone:
- 29E4.G7
- Clonality:
- Monoclonal
- Conjugate:
- DyLight™ 680
- Concentration:
- 1 mg/ml
- Extra Details:
- The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.
- Formulation:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Host:
- Mouse
- Immunogen:
- This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.
- Isotype:
- IgG2a, IgG2a, kappa
- Physical State:
- Lyophilized
- Reactivities:
- Label/Epitope-tag
- Shipping Conditions:
- Ambient
- Specificity:
- This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
- Storage Conditions:
- 2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Type:
- Antibody: Tagged Antibodies
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=200-344-383
