DYKDDDDK Tag (Anti-FLAG[R]) Antibody Biotin Conjugated
Product Sizes
100 ug
200-306-383-100UG
About this Product
- SKU:
- 200-306-383
- Additional Names:
- mouse anti-FLAG™ tag biotin conjugation, Enterokinase Cleavage Site (ECS), biotin conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
- Application:
- ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
- Buffer:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
- CE/IVD:
- RUO
- translate.label.attr.clone:
- 29E4.G7
- Clonality:
- Monoclonal
- Conjugate:
- Biotin
- Concentration:
- 1 mg/ml
- Extra Details:
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG™ and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
- Formulation:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Host:
- Mouse
- Immunogen:
- This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.
- Isotype:
- IgG2a, IgG2a, kappa
- Physical State:
- Lyophilized
- Reactivities:
- Label/Epitope-tag
- Shipping Conditions:
- Ambient
- Specificity:
- This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
- Storage Conditions:
- 2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Type:
- Antibody: Tagged Antibodies
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=200-306-383
