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Antibodies

Alkaline Phosphatase Antibody

Product Sizes
2 ml
100-4135-2ML
About this Product
SKU:
100-4135
Additional Names:
ALP1|rabbit anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody
Application:
ELISA, Immunofluorescence, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
85 mg/ml
Extra Details:
In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Alkaline Phosphatase catalytic activity is phosphate monoester + H2O = an alcohol + phosphate.
Format:
Antiserum
Formulation:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Alkaline Phosphatase [Calf intestine]
Physical State:
Lyophilized
Reactivities:
Bovine
Shipping Conditions:
Ambient
Specificity:
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alkaline Phosphatase [Calf intestine]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined. No reaction wa
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence of Anti-Alkaline Phosphatase Antibody. Localization of apical and basolateral proteins in the small intestine of Rab11a IKO mice at P21. (B) Localization of the apical proteins alkaline phosphatase (AlP) and aminopeptidase N (APN) in control (Ctrl) and IKO cells at P21. Merged figures of AlP and APN are shown on the right. Intracellular vacuoles of AlP and APN are indicated by arrowheads. Basolateral localization is indicated by arrows. Scale bars: 10 um. N, nucleus. Fig 3. PMID: 25527643.
Western Blot of Anti-Alkaline Phosphatase Antibody. Quantification of a starvation marker and protein levels. (A) Western blot analysis of protein levels in crude extracts from the small intestines of control (Ctrl) and IKO mice at P5. Small intestine extracts were analyzed by Western blot analysis using antibodies against various apical proteins (DPPIV and AlP), (additional not shown). Fig 5. PMID: 25527643.