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Antibodies

Cul4A Antibody

Product Sizes
100 ul
100-401-A04-100UL
About this Product
SKU:
100-401-A04
Additional Names:
CUL4A|rabbit anti-Cul4A Antibody, 2810470J21Rik antibody, AW495282 antibody, CUL 4A antibody, CUL4A antibody, Cul4a protein antibody, MGC36573 antibody, MGC64071 antibody, CUL, Cullin
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
None
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
85 mg/ml
Extra Details:
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 4a is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul4A serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul4A may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul4A also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.
Format:
Antiserum
Host:
Rabbit
Immunogen:
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near amino acids 1-25 of Human Cul4A coupled to KLH.
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 4A. Cross reactivity is expected against mouse Cul4A based on a high degree of sequence homology. Cross reactivity with other human cullins may occur.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.
Immunohistochemistry. Rockland's anti-CUL4 antibody was diluted 1:500 to detect CUL4 in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Western Blot of Rabbit Anti-Cul4 Antibody. Lane 1: Opal Pre-Stained Ladder (p/n MB-210-0500). Lane 2: human Testis. Lane 3: human skeletal muscle. Load: 35ug lysate per lane. Primary Antibody: Anti-Cul4 at 1:500 overnight at 2-8°C. Secondary Antibody: Goat anti-rabbit IgG HRP (p/n 611-103-122) at 1:70,000 at RT for 30 mins. Expect: ~87.6kDa.
Western Blot of Rabbit Anti-Cul4 Antibody. Lane 1: Opal Pre-Stained Ladder (p/n MB-210-0500). Lane 2: A431 whole cell lysate (p/n W09-000-361). Lane 3: U2OS whole cell lysate (p/n W09-001-374). Lane 4: HeLa whole cell lysate (p/n W09-000-364). Load: 35ug lysate per lane. Primary Antibody: Anti-Cul4 at 1:500 overnight at 2-8°C. Secondary Antibody: Goat anti-rabbit IgG HRP (p/n 611-103-122) at 1:70,000 at RT for 30 mins. Expect: ~87.6kDa.