ELISA Pro: Human Latent TGF-B Beta1
Product Sizes
1 plate
3550-1HP-1-1PLATE
About this Product
- SKU:
- 3550-1HP-1
- Application:
- ELISA
- Extra Details:
- The human latent TGF-B Beta1 ELISA Pro kit provides all necessary reagents and solutions required to quantify human latent TGF-B Beta1 in solution, e.g. in cell culture supernatant, and serum/plasma samples. The kit contains an ELISA plate pre-coated with monoclonal capture antibody, biotinylated detection antibody, Streptavidin-HRP, TMB substrate, ELISA standard and ELISA diluent. The ELISA diluent prevents false positive read-outs which may be caused by heterophilic antibodies found in serum and plasma samples. TGF-B Beta1 is secreted in a latent, biologically inactive form, non-covalently linked with a Latency Associated Protein (LAP). The monoclonal antibodies MT593 and MT517-biotin bind to the LAP entity of human latent TGF-B Beta1. This enables direct quantification of latent TGF-B Beta1 in ELISA with no need of sample pre-treatment. For quantification of latent TGF-B Beta1 in blood, the use of plasma is recommended since serum contains high levels of latent TGF-B Beta1 released from platelets during sample preparation. Plasma can be obtained using EDTA, citrate, or heparin as anti-coagulants. To minimize the platelet conten in the sample, and additional centrifugation of plasma at 10,000 x g for 10 minutes is recommended. The ELISA does not detect human latent TGF-B Beta2 or -B Beta3 or bovine latent TGF-B Beta1.|Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.|This ELISA Pro kit is intended for the quantification of human latent Transforming Growth Factor-B Beta1 (TGF-B Beta1) in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.
- Format:
- Pro
- Immunogen:
- TGF-β1 (Latent TGF-β1)
- Range:
- 0.4-40 pM
- Reactivities:
- Human, Non-human Primate
- Shipping Conditions:
- Blue Ice
- Specificity:
- This kit is based on a matched pair of mAbs specific for the Latency Associated Protein (LAP) of human latent TGF-B Beta1.
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Mabtech
- Type:
- Assay Kits: ELISAs
