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Antibodies

Mouse Anti-HA Tag Antibody [2-G10], Mouse monoclonal Antibody

Product Sizes
100uL
M1008-1-M1008-1
About this Product
SKU:
M1008-1
Application:
WB, IP, IF-Cell
Buffer:
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
2-G10
Clonality:
Mouse monoclonal Antibody
Concentration:
2 mg/mL.
Control:
Recombinant protein with HA tag, HA-Gag transfected Hela cells.
Format:
Liquid
Host:
Mouse
Immunogen:
Synthetic peptide corresponding to HA tag conjugated to KLH.
Isotype:
IgM
Purification:
Protein G affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at -20℃. Stable for 12 months from date of receipt.
Supplier:
Huabio
Western blot analysis on recombinant protein with HA tag using anti-HA tag Mouse mAb (Cat. # M1008-1).
HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with M1008-1 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using ET1611-49 at 1/500 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature. Lane 1: HA Tag fusion protein lysate (input). Lane 2: M1008-1 IP in HA Tag fusion protein lysate. Lane 3: Mouse IgG instead of M1008-1 in HA Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST
Immunocytochemistry analysis of HA-Tag transfected Hela cells labeling HA Tag with Mouse anti-HA Tag antibody (M1008-1) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-HA Tag antibody (M1008-1) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor[TM] 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.