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Antibodies

Mouse Anti-Lysozyme Antibody [A5A8-R], Recombinant Mouse monoclonal Antibody

Product Sizes
100uL
HA601229-HA601229
About this Product
SKU:
HA601229
Application:
WB, IHC-Paraffin
Buffer:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
A5A8-R
Clonality:
Recombinant Mouse monoclonal Antibody
Concentration:
1 mg/mL.
Control:
HL-60 cell lysate, U-937 cell lysate, human spleen tissue, human stomach cancer tissue.
Format:
Liquid
Host:
Mouse
Immunogen:
Recombinant protein within Human Lysozyme aa 1-141 / 148.
Isotype:
IgG1
Molecular Weight:
Predicted band size: 17 kDa
Purification:
Protein A affinity purified.
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Western blot analysis of Lysozyme on different lysates with Mouse anti-Lysozyme antibody (HA601229) at 1/1,000 dilution. Lane 1: HL-60 cell lysate Lane 2: U-937 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 17 kDa Observed band size: 14 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601229) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-Lysozyme antibody (HA601229) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601229) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Mouse anti-Lysozyme antibody (HA601229) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601229) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.