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Antibodies

Mouse Anti-Beta Actin Antibody [A2-F6], Mouse monoclonal Antibody

Product Sizes
100uL
EM21002-EM21002
About this Product
SKU:
EM21002
Application:
WB, IF-Cell, IHC-Paraffin, FC
Buffer:
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
A2-F6
Clonality:
Mouse monoclonal Antibody
Concentration:
2 mg/mL.
Control:
Hela cell lysate, THP-1 cell lysate, HepG2 cell lysate, MCF-7 cell lysate, PC-12 cell lysate, PMVEC
Format:
Liquid
Host:
Mouse
Immunogen:
Synthetic peptide (KLH-coupled) within human Beta-actin aa 1-50.
Isotype:
IgG1
Molecular Weight:
Predicted band size: 42 kDa
Purification:
Protein A affinity purified.
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Western blot analysis of Beta Actin on Hela cell lysates with Mouse anti-Beta Actin antibody (EM21002). Hela cell lysates at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM21002) at serial dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Beta Actin on different lysates with Mouse anti-Beta Actin antibody (EM21002) at 1/40,000 dilution. Cell lysates at 10 µg/Lane, tissue lysates at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM21002) at 1/40,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Beta Actin on different lysates with Mouse anti-Beta Actin antibody (EM21002) at 1/20,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HepG2 cell lysate Lane 3: PMVEC cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: L929 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM21002) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.
ICC staining of Beta Actin in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (EM21002, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution.
ICC staining of Beta Actin in A549 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (EM21002, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution.