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Antibodies

Anti-Talin (Ser-425), Phosphospecific Antibody

Product Sizes
100 ul
TP4171-100UL
About this Product
SKU:
TP4171
Additional Names:
TLN1
Application:
Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Ser-425 - Phosphorylated
Extra Details:
Our Talin (Ser-425) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Talin (Ser-425) and is antigen affinity purified. It is great for use in WB, ICC.
Format:
Antigen Affinity Purified
Gene Details:
TLN1
Host:
Rabbit
Immunogen:
Phospho-Talin (Ser-425) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-425 in human Talin 1. This sequence is conserved in rat, mouse, and chicken Talin 1, as well as in Talin 2 (Ser-428).
Isotype:
IgG
Molecular Weight:
240
Physical State:
Liquid
Reactivities:
Amphibian, Avian, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 240 kDa* protein corresponding to the molecular mass of Talin on SDS-PAGE immunoblots of human A431, rat PC12, and rabbit fibroblast cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Immunocytochemical labeling of Talin phosphorylation relative to F-actin in chick fibroblasts. The cells were labeled with rabbit polyclonal Talin (Ser-425) antibody (TP4171), then the antibody was detected using appropriate secondary antibody (Green). This labeling is compared to F-actin staining (Red, Left). (Image provided by Dr. Gianluca Gallo at Drexel University).
Western blot of rat PC12 cells stimulated with Calyculin A (100 nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin (Ser-425) (lanes 3 & 4) antibodies.
Representative WB of whole cell lysates from sh-SCR and sh-ARRB1 HOFs stimulated or not with ET-1 (5 min) and probed with antibodies to active or total IntB Beta1, b-arr1, pTalin (cat. TP4171) and Talin. Image from publication CC-BY-4.0. PMID: 38777849