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Antibodies

Anti-Trop-2/TACSTD2 (Extracellular region) Antibody

Product Sizes
100 ul
TM0051-100UL
About this Product
SKU:
TM0051
Additional Names:
Tumor-associated calcium signal transducer 2; Trop-2; Membrane component chromosome 1 surface marker 1; GA733-1; TACSTD2; M1S1; TROP2
Application:
Immunocytochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
M005
Clonality:
Monoclonal
Conjugate:
- GOmAb
Extra Details:
Our Trop-2/TACSTD2 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Trop-2/TACSTD2 (Extracellular region) and is Protein G purified. It is great for use in WB, ICC, IP.
Format:
Protein G Purified
Gene Details:
TACSTD2
Host:
Mouse
Immunogen:
Clone M005 was generated from a proprietary antigen related to the extracellular region of human Trop-2 from the A431 epidermoid carcinoma cell line.
Isotype:
IgG1
Molecular Weight:
50-65
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Blue Ice
Specificity:
Clone M005 detects a 50-65 kDa* band corresponding to the molecular mass of Trop-2 on SDS-PAGE immunoblots of native human A431, MDA-MB-231, and MCF-7 cell lysates. The antibody also detects denatured Trop-2, but with less affinity. Lower dilutions of the antibody should be used for denatured western blot. Clone M005 can be used for western blot, immunocytochemistry, ELISA, and immunoprecipitation
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Monoclonal Antibody
Western blotting for selected HGSC-specific EV-associated proteins on U fractions of 11 patients and Caov-3 and Kuramochi HGSC cell lines. Caov-3 and Kuramochi cells are used as negative controls for macrophage markers MRC1, CD68, and FCGR1A. For TACSTD2 (cat. TM0051, 1:500), Caov-3 serves as a positive control, while Kuramochi serves as a negative control. Image from publication CC-BY-4.0. PMID: 38490958
Representative Standard Curve using mouse monoclonal anti-Trop-2 (TM0051) for ELISA capture of human recombinant Trop-2 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
Immunocytochemical labeling of Trop-2 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Trop-2 (clone M005). The antibody was detected using goat anti-mouse DyLight® 594.