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Antibodies

Anti-Slingshot-1L (Ser-978), Phosphospecific Antibody

Product Sizes
100 ul
SP3901-100UL
About this Product
SKU:
SP3901
Additional Names:
SSH1L
Application:
Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Ser-978 - Phosphorylated
Extra Details:
Our Slingshot-1L (Ser-978) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Slingshot-1L (Ser-978) and is antigen affinity purified. It is great for use in WB, ICC.
Format:
Antigen Affinity Purified
Gene Details:
SSH1
Host:
Rabbit
Immunogen:
Slingshot-1L (Ser-978) antibody was generated from a phospho-peptide that included amino acids surrounding serine 978 in human SSH1L. This sequence has high homology to the conserved site in rat, mouse, chicken, and zebrafish SSH1L. The sequence also has high homology to the conserved site in SSH2L (Ser-1259).
Isotype:
IgG
Molecular Weight:
150
Physical State:
Liquid
Reactivities:
Avian, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 150 kDa* full length recombinant human SSH1L phosphoprotein and this reactivity is eliminated with lambda phosphatase treatment. The antibody also detects SSH1L on immunoblots of rat PC12 and mouse C2C12 cells that were stimulated with calyculin A.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
DU-145 cells were transfected with control (Ctrl siRNAs) or AKAP2 specific siRNAs (siRNA1 and siRNA2). 72 h after transfection, cell lysates were loaded on SDS-PAGE gels and analyzed by Western blot. Phospho-SSH1 was detected using antibodies recognizing phospho-serines 937 and 978 (cat. SP3901). The amounts of SSH1 (cat. SP1711) and AKAP2 were detected using specific antibodies, as indicated. Image from publication CC-BY-4.0. PMID: 37827203
Western blot of human recombinant SSH1L untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1L (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1L (Ser-978) (lanes 3, 4, 7, & 8).
Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-SSH1L (C-term.) and anti-SSH1L (Ser-978) antibodies, then detected using appropriate secondary antibody conjugated to Cy3 (Right panel). Phase image of corresponding PC12 cells (Left panel).