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Antibodies

Anti-nNOS (C-terminal region) Antibody

Product Sizes
100 ul
NM4011-100UL
About this Product
SKU:
NM4011
Additional Names:
nNOS, Constitutive NOSb, neuronal nitric oxide synthase, NCNOS
Application:
Immunocytochemistry, Immunohistochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
M401
Clonality:
Monoclonal
Conjugate:
- GOmAb
Extra Details:
Our nNOS (C-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat nNOS (C-terminal region) and is Protein A purified. It is great for use in WB, ICC, IHC.
Format:
Protein A Purified
Gene Details:
NOS1
Host:
Mouse
Immunogen:
Clone (M401) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human nNOS. This human nNOS sequence has high homology with similar regions in rat and mouse nNOS.
Isotype:
IgG2a
Molecular Weight:
155
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 155 kDa* protein on SDS-PAGE immunoblots of rat and mouse brain tissue lysates, as well in the rat pituitary cell line GC.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Monoclonal Antibody
Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NM4011), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (lanes 3 & 4).