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Antibodies

Anti-MuRF1 (C-terminal region) Antibody

Product Sizes
100 ul
MP3401-100UL
About this Product
SKU:
MP3401
Additional Names:
Tripartite motif 63,TRIM63, SMRZ, IRF, RNF28, Muscle RING finger, MURF-1
Application:
Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Our MuRF1 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat MuRF1 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.
Format:
Antigen Affinity Purified
Gene Details:
TRIM63
Host:
Rabbit
Immunogen:
MuRF1 (C-terminal) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal half of human MuRF1. This peptide sequence is highly conserved in rat and mouse MuRF1, and has 50% homology to MuRF2 (TRIM-55).
Isotype:
IgG
Molecular Weight:
30/38
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects 38 and 30 kDa* proteins corresponding to the apparent molecular mass of MuRF1 isoforms on SDS-PAGE immunoblots of mouse C2C12 cells, and detects a 38 kDa band in mouse heart and muscle tissue.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Immunocytochemical labeling of MuRF1 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1 antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (MX3405).
Western blot analysis of MuRF1 (cat. MP3401), Atrogin-1 (cat. AP2041), FoxO3a, p-AKT (Ser473), and total AKT in the soleus muscle of control, HU, and HU+TUDCA mice. Image from publication CC-BY-4.0. PMID: 37525907
Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1 (C-terminal region) (lanes 1 & 2) or anti-Atrogin-1 (lanes 3 & 4).
Western blotting analysis of the expression of Atrogin-1 (cat. AP2041, 1:1000) and MuRF1 (cat. MP3401, 1:1000) proteins in the soleus muscles after HS. Image from publication CC-BY-4.0. PMID: 37344807
Western blot showing MuRF-1( cat MP3401, 1:500) and atrogin-1 (cat. AP2041), 1:500) levels in C2C12 myoblasts differentiated for 4-5 days were incubated with 200 uM UDCA for 72 h. GAPDH was used as a loading control. Image from publication CC-BY-4.0. PMID: 37237400