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Antibodies

Anti-eEF2K (C-terminus) Antibody

Product Sizes
100 ul
EP4661-100UL
About this Product
SKU:
EP4661
Additional Names:
eEF-2, eEF-2K, CaMK-III, eukaryotic elongation factor
Application:
Immunocytochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Our eEF2K (C-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus eEF2K (C-terminus) and is antigen affinity purified. It is great for use in WB, ICC, IP.
Format:
Antigen Affinity Purified
Gene Details:
EEF2K
Host:
Rabbit
Immunogen:
eEF2K (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human eEF2K. This sequence is well conserved in rat, mouse, chicken, and fish eEF2K, and has low homology to other proteins.
Isotype:
IgG
Molecular Weight:
105
Physical State:
Liquid
Reactivities:
Amphibian, Avian, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of human A431, HeLa, and Jurkat cells. The antibody also detected eEF2K in immunoprecipitations in HeLa cells and immunofluorescent labeling in A431 cells.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Immunocytochemical labeling of eEF2K in paraformaldehyde fixed and NP-40 permeabilized A431 cells. The cells were labeled with rabbit polyclonal anti-eEF2K (EP4661). The antibody was detected using goat anti-rabbit DyLight® 594.
Western blot image of eEF2K in human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells, and immunoprecipitation of eEF2K from HeLa cell lysate using anti-eEF2K (C-terminus) antibody (lanes 4 & 6). Negative control immunopreciptations with no antibody are shown in lane 5 and 7. The blots were probed with anit-eEF2K (C-terminus) (lanes 1-5) or with anti-eEF2K (N-terminal) antibody (lanes 6 & 7).