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Antibodies

Anti-CD47 (Extracellular region) Antibody

Product Sizes
100 ul
CM0541-100UL
About this Product
SKU:
CM0541
Additional Names:
Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Leukocyte surface antigen CD47, MER6
Application:
Immunocytochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
M054
Clonality:
Monoclonal
Conjugate:
- GOmAb
Extra Details:
Our CD47 (Extracellular region) Antibody mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human CD47 (Extracellular region) Antibody and is Protein G purified. It is great for use in WB(Nat), ICC, IP.
Format:
Protein G Purified
Gene Details:
CD47
Host:
Mouse
Immunogen:
Clone (M054) was generated from a recombinant protein that included the extracellular region of human CD47 protein.
Isotype:
IgG1
Molecular Weight:
45-55
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Blue Ice
Specificity:
Clone M054 mouse monoclonal antibody detects native human CD47, as well as aldehyde-fixed CD47. The antibody works for staining in immunocytochemistry, capture and detection in ELISA, native immunoprecipitation and western blot. The antibody detects a 45 to 55 kDa band in native western blots of A431, BEAS-2B, MCF7, NCI-H446, NCI-H28, and NCI-H2052 cell lines.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Monoclonal Antibody
Representative Standard Curve using mouse monoclonal anti-CD47 (CM0541) for ELISA capture of human recombinant CD47 extracellular region with a His-tag. Captured protein was detected by suitable anti-His-tag antibody followed by appropriate secondary antibody HRP conjugate.
Immunocytochemical labeling of CD47 in aldehyde fixed human MCF7 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-CD47 (CM0541). The antibody was detected using goat anti-mouse DyLight® 594.
Native western blot of human BEAS-2B lung cells (lane 1), NCI-H446 lung cancer cells (lane 2), lung mesothelioma cells: NCI-H28 (lane 3), and NCI-H2052 (lane 4). The blot was probed with mouse monoclonal anti-CD47 (CM0541) at 1:500.