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Antibodies

Anti-AIFM1 (Ser-116), Phosphospecific Antibody

Product Sizes
100 ul
AP5501-100UL
About this Product
SKU:
AP5501
Additional Names:
PDCD8, AIF1, Apoptosis inducing factor 1, mitochondrial; Program cell death protein 8; striatal AIF
Application:
Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Ser-116 - Phosphorylated
Extra Details:
Our AIFM1 (Ser-116) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human AIFM1 (Ser-116) and is antigen affinity purified. It is great for use in WB.
Format:
Antigen Affinity Purified
Gene Details:
AIFM1
Host:
Rabbit
Immunogen:
Phospho-AIFM1 (Ser-116) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-116 in human AIFM1. This site is conserved in rat and mouse AIFM1, but the amino acids surrounding the site are not well conserved. The site is not found in AIFM2 and AIFM3.
Isotype:
IgG
Molecular Weight:
57/62/66
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Blue Ice
Specificity:
The antibody was cross adsorbed to unphosphorylated AIFM1 (Ser-116) peptide before affinity purification using phospho-AIFM1 (Ser-116) peptide. This antibody antibody detects a triplet at 66, 62, and 57 kDa* protein on SDS-PAGE immunoblots of human Jurkat cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment to dephosphorylated AIFM1.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Western blot image of human jurkat cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM, 30 min.) (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-AIFM1 (C-terminal region) at 1:500 (lanes 1 & 2) and anti-AIFM1 (Ser-116) phospho-specific antibody at 1:1000 (lanes 3 & 4).
Western blotting shows KEAP1-PGAM5-AIFM1 pathway protein level regulation by ALT. SKOV-3 cells were treated with different concentrations (0, 16, 32 and 64 uM) of ALT for 24 h. Specifically AIFM1, P-AIFM1(Ser-116) (cat. AP5501), KEAP1, Nrf2, and PGAM5 levels were detected. B-Actin used as a control. Image from publication CC-BY-4.0. PMID: 37712005
Western blot analysis of protein expression of ASIC1, KEAP1, PGAM5, and AIFM1 phosphorylated at Ser116 (cat. AP5501, 1:1000) in NPCs after treatment for 30 min with 20 nM Psalmotoxin 1 (PcTx1) followed by 24 h of treatment with H2O (control) or 100 uM TBHP. Image from publication CC-BY-4.0. PMID: 38540291