Elastase
Product Sizes
0.05 mg
16-14-051200-0.05MG
0.1 mg
16-14-051200-0.1MG
1 mg
16-14-051200-1MG
About this Product
- SKU:
- 16-14-051200
- Additional Names:
- Host Defense, Antimicrobial, Inflammation, Cancer, NETosis, Emphysema, COPD, SARS-CoV-2, Rheumatoid Arthritis, In Vitro Diagnostic|Neutrophil Elastase, HNE, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, Medullasin, PMN elastase, HLE, ELANE, ELA2
- Buffer:
- Salt-free lyophilized solid.
- CAS Number:
- 9004-06-2
- CE/IVD:
- RUO
- Extra Details:
- Neutrophil elastase (NE), a serine protease stored in azurophilic granules of neutrophils, plays critical roles in host defense and tissue remodeling. As a member of the chymotrypsin superfamily, NE hydrolyzes elastin, collagen, and proteoglycans through its catalytic triad (His-Asp-Ser), enabling pathogen clearance via microbial membrane disruption. Beyond direct bactericidal activity, NE modulates immune responses by activating matrix metalloproteinases (MMPs), inducing pro-inflammatory cytokines (TNF-A Alpha, IL-1B Beta, IL-8), and promoting neutrophil extracellular trap (NET) formation. However, unchecked NE activity drives extracellular matrix degradation, contributing to chronic inflammatory and degenerative diseases. NE dysregulation is central to pulmonary emphysema, particularly in A Alpha1-antitrypsin deficiency, where insufficient protease inhibition leads to alveolar destruction. In chronic obstructive pulmonary disease (COPD), NE excess exacerbates airway inflammation and mucus hypersecretion, perpetuating lung parenchymal damage. NE also directly injures cartilage in rheumatoid arthritis by degrading collagen and activating fibroblast-like synoviocytes, amplifying joint inflammation. Additionally, elevated NE levels correlate with cystic fibrosis progression, acute respiratory distress syndrome (ARDS), and severe COVID-19, where it enhances viral entry and cytokine storms. Balancing NE's protective and pathogenic roles remains a clinical challenge, necessitating therapies that preserve antimicrobial function while preventing tissue destruction.
- Formulation:
- Reconstitution: After centrifugation, we suggest addition of 50 mM Na Acetate, pH 5.5, with 150 mM NaCl to the original volume, followed by gentle mixing to ensure adequate homogenization.
- Molecular Weight:
- 29,500 Da
- Physical State:
- Lyophilized
- Purity:
- ≥95%
- Purification:
- Liquid Chromatography Methods
- Shipping Conditions:
- Blue Ice
- Source:
- Neutrophils shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Athens Research & Technology Inc.
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
