Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin)

Product Sizes
100 ug
ARG66714-100UG
500 ug
ARG66714-500UG
Academic Pricing
Please contact us for academic pricing.
Contact us
About this Product
SKU:
ARG66714
Additional Names:
HMG-1; High mobility group protein B1; High mobility group protein 1; HMG1; SBP-1; HMG3
Application:
Block/Inhibit/Neutralise, ELISA, Flow Cytometry, Western Blot
Buffer:
PBS (pH 7.4)
CE/IVD:
RUO
translate.label.attr.clone:
SQab20175
Clonality:
Monoclonal
Concentration:
0.5 mg/ml
Extra Details:
HMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
Host:
Mouse
Immunogen:
Human HMGB1.
Isotype:
IgM, kappa
Purification:
Affinity Purified
Reactivities:
Bovine, Human, Mouse
Shipping Conditions:
Blue Ice
Specificity:
This antibody could specifically neutralize HMGB1 induced pathway but not HMGB2 tested by TNF alpha releasing assay.
Storage Conditions:
2[o]C to 8[o]C or -20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Monoclonal Antibody
Neutralization assay: HCMEC in the presence of ARG66714 anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin) or ARG65345 Mouse IgG Isotype Control antibody.
ELISA: 2 ug/ml dilution of recombinant human HMGB1, bovine HMGB1 and recombinant human HMGB2 proteins (left to right) were coated onto ELISA plate. ARG66714 anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin) at 1:3500 dilution were used for detecting HMGB1.
Flow Cytometry: HuH-7 cells (black) and HuH-7 shHMGB1 cells (red) stained with ARG66714 anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin) at 1:500 dilution.
Neutralization assay: Mouse were injected with E. coli (3 x 10^9 CFU/kg) intraperitoneally. After 3 hours, antibodies (15 mg/kg) were given by i.p. injection. Mouse sera were collected 24 hours later and examined the level of IL6.
Neutralization assay: Cell culture medium from THP1 cells treated with 10 ug of isotype control mouse IgM, plus 2ug/ml of rhHMGB1 or not as control. THP1 cells treated with 2ug/ml of rhHMGB1 plus 0.1 ug, 1 ug or 10 ug of ARG66714 anti-HMGB1 Neutralizing antibody [SQab20175] (low endotoxin) (left to right) as assay groups. TNF alpha concentration was tested by ARG80120 Human TNF alpha ELISA Kit.