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Antibodies

anti-FFAR2 / GPR43 antibody

Product Sizes
100 ul
ARG66475-100UL
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG66475
Additional Names:
FFA2R; Free fatty acid receptor 2; G-protein coupled receptor 43; GPR43
Application:
IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for short chain free fatty acids and may be involved in the inflammatory response and in regulating lipid plasma levels. [provided by RefSeq, Apr 2009]
Host:
Rabbit
Immunogen:
KLH-conjugated synthetic peptide within the C-terminal region of Human FFAR2 / GPR43.
Isotype:
IgG
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Polyclonal Antibody
Immunofluorescence: Formalin-fixed THP-1 cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at RT. Cells were stained with ARG66475 anti-FFAR2 / GPR43 antibody (red) in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. DAPI (blue) for nuclear staining.
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then stained with ARG66475 anti-FFAR2 / GPR43 antibody at RT and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot: THP-1 whole cell lysate stained with ARG66475 anti-FFAR2 / GPR43 antibody.