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Antibodies

anti-Akt 1 + Akt 2 + Akt 3 phospho (Tyr315 / 316 / 312) antibody

Product Sizes
50 ul
ARG51805-50UL
100 ul
ARG51805-100UL
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG51805
Additional Names:
Protein kinase B alpha; Proto-oncogene c-Akt; RAC; PKB alpha; RAC-ALPHA; CWS6; PRKBA; AKT; PKB; RAC-PK-alpha; PKB-ALPHA; RAC-alpha serine/threonine-protein kinase; EC 2.7.11.1; Protein kinase B
Application:
IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
Host:
Rabbit
Immunogen:
Peptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y(p)-L-A) derived from Human AKT1/AKT2/AKT3.
Isotype:
IgG
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Polyclonal Antibody
Western blot: Extracts from HepG2 cells untreated or treated with EGF stained with ARG51805 anti-Akt 1 + Akt 2 + Akt 3 phospho (Tyr315 / 316 / 312) antibody.
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51805 anti-Akt 1 + Akt 2 + Akt 3 phospho (Tyr315 / 316 / 312) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51805 anti-Akt 1 + Akt 2 + Akt 3 phospho (Tyr315 / 316 / 312) antibody.
Western blot: Extracts from 3T3 cells, treated with EGF or calf intestinal phosphatase (CIP), stained with ARG51805 anti-Akt 1 + Akt 2 + Akt 3 phospho (Tyr315 / 316 / 312) antibody.