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Vector Laboratories

Vector Laboratories develops, manufactures and markets high quality labeling and detection reagents for immunohistochemistry (IHC), immunofluorescence, glycobiology, lectin research, and the study of DNA/RNA. Allowing precise visualisation of a wide range of cells and tissues, these tools have been pivotal to accelerate disease and therapeutics research for over forty years. Researchers frequently turn to us for Vector Laboratories’ many innovative technologies. Highly popular among these, VECTASTAIN® ABC Kits rely on the avidin-biotin interaction to deliver exceptional assay sensitivity at low cost, making them a preferred choice for applications such as IHC, immunocytochemistry, protein and nucleic acid blot, in situ hybridization, and ELISA.

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Vector Laboratories
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Veterinary Reagents

ImmPRESS® VR Polymer Detection Kits for farm, pet and lab animal tissues

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Vector® TrueVIEW™ Autofluorescence Quenching Kit

Dramatically Reduce Autofluorescence

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Vector Laboratories

Introducing VECTASHIELD® Vibrance™ Antifade Mounting Media

VECTASHIELD Vibrance products are completely new formulations of curing antifade mounting media for immunofluorescence applications. These newest additions to our VECTASHIELD portfolio were developed with customer feedback to improve on parameters such as ease of use and retention of specific staining intensity over time. VECTASHIELD Vibrance Antifade Mounting Media are new tools to help investigators see more and do more with each experiment. 

Why VECTASHIELD Vibrance Mounting Media?

  • Superior antifade/anti-photobleaching properties across the spectrum
  • Compatibility with commonly used fluorophores
  • View sections one hour after mounting
  • No tone or autofluorescent background, even after curing
  • Room temperature storage of slides with extended archiving time
  • Minimal bubble formation, even after several weeks storage
  • Curing formulations with choice of counterstain (DAPI) or no counterstain
  • 12 month product expiration date

Plus the same features you have come to expect of VECTASHIELD® brand:

  • Ideal refractive index
  • Easy to use
  • Ready to use, requires no warming
  • No sealing of coverslips required (curing formulations)

Easy Application and Use of VECTASHIELD Vibrance

Vector Laboratories
Immunofluorescent, staining, vectashield, mounting,
Excellent Antifade Properties Across the Spectrum, with Commonly Used Fluorophores

VECTASHIELD Vibrance Mounting Media provide excellent protection against fading across the visible spectrum, even under far-red wavelengths. They are compatible with most commercially available fluorophores.

Fluorophore Excitation/Emission (nm) Vectashield Vibrance (H-1700) Vectashield Vibrance with DAPI (H-1800)
Fluorescein 495/515 ++++ ++++
Alexa Fluor® 488 490/525 +++ +++
Dylight® 488 493/518 +++ +++
Cy® 3 550/570 ++++ ++++
Alexa Fluor® 594 590/617 ++++ ++++
Dylight® 594 593/618 ++++ ++++
Cy® 5 649/670 ++++ ++++
Alexa Fluor® 647 650/665 ++++ ++++
Dylight® 649 652/672 ++++ ++++

Viewable in as Little as One Hour After Mounting – No Need to Wait 24 Hours

Competitor immunofluorescence workflows recommend slides be viewed 24 hours after mounting. VECTASHIELD Vibrance Mounting Media, however, allow same-day viewing to accelerate discovery without sacrificing signal intensity or retention.

Vector Laboratories

Improved retention of fluorescence with VECTASHIELD Vibrance Mounting Medium. Serial sections of human colon tissue (FFPE) stained for cytokeratin (mouse primary antibody AE1/AE3) and detected with fluorescein-conjugated horse anti-mouse IgG secondary antibody (FI-2000). One hour after mounting with either VECTASHIELD Vibrance (top row) or a competitor mounting medium (bottom row), the sections were imaged at the intervals indicated. Note the improved retention of fluorescent signal for sections mounted with VECTASHIELD Vibrance Mounting Medium.

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