Research areas for which we frequently recommend Mabtech’s kits include infectious disease, cancer, allergy, transplantation and vaccine monitoring, and we also endorse Mabtech antibodies for cardiovascular and veterinary research.
Researchers with an interest in immune monitoring regularly turn to us as a source of Mabtech ELISpot kits, especially those who have invested in Mabtech’s IRIS™ ELISpot reader. One question we’re often asked is ‘what are the main differences between Mabtech’s ELISpot, FluoroSpot and ELISA kits?’. While all these products are based on the same high-quality Mabtech antibodies, they differ in terms of configuration.
Mabtech ELISAs are performed within a standard microplate, typically resulting in a colorimetric readout, whereas during a Mabtech ELISpot assay the microplate wells contain a PVDF membrane coated with a capture antibody to quantify cytokine-releasing cells. The FluoroSpot assay builds on the ELISpot principle by providing the capacity to analyze the secretion of multiple analytes from the same cell.