PROTEOMICS OF UBIQUITIN
Post translational modification (PTM) of proteins plays a fundamental role in their function. Attachment of ubiquitin to epsilon (ε) amino group of lysine in target proteins represent the major PTM. In addition to the attachment of ubiquitin (ubiquitylation) to lysines, other ubiquitin-like-proteins (UBLs) such as SUMO (sumoylation), NEDD8 (neddylation) and ISG15 (isgynylation) are equally well represented PTMs of proteins. Ubiquitylation of proteins is a hallmark of proteins, provides clues to many diseases, and acts as a biomarker. Modern drug discovery and personalized medicine relies heavily upon proteomics to identify these signatures. A variety of methods are available to detect ubiquitin or UBL PMTs of proteins. We encourage you to contact our senior staff to learn about how LifeSensors' PTM technology can help in your research and drug discovery process. One of the best ways to perform ubiquitin and UBL proteomics is to enrich ubiquitylated proteins with LifeSensors' TUBE technologies and perform mass spectrometry studies. Lifesensors offers state of the art services to determine sites of ubiquitylation and UBLs by utilizing a combination of TUBE and UbiSight antibody applications. The combination of these two powerful technologies enables researchers to unravel the proteome and ubiquitome.
If You Are Not Using VU-1 anti-ubiquitin Antibody, You Are Losing Data
Anyone researching the Ubiquitin Proteasome System (UPS) needs to use VU-1 for all their assays. This is the most selective and sensitive antibody on the market, and could be the difference between a publication and a messy blot.
TUBEs (Tandem Ubiquitin Binding Entities) range