VectaPlex Antibody Removal Kit
Product Sizes
125 reactions / 1 kit
VRK-1000-125REACTIONS/1KIT
1 kit
VRK-1000-1KIT
About this Product
- SKU:
- VRK-1000
- Application:
- Immunofluorescence
- Extra Details:
- Your path to simplified multiplexing starts with VectaPlex™. This kit is designed to remove antibodies and other non-covalently bound detection reagents from FFPE tissue sections in immunofluorescence and tyramide signal amplification staining workflows. Benefits include: -Obtain more spatial biomarker information from each tissue section. - Detect 6+ targets in a single tissue using two-color fluorescent imaging and standard IF reagents. - Multiplex at room temperature with minimal tissue damage or loss of antigenicity. Preserve precious sample in IF and TSA, including sensitive tissues such as lung, skin, and breast. - Stain with multiple primaries from the same species and a single secondary without cross-reactivity. - Reduce the need for optimization of the sequence in which antibodies are bound during TSA. Kit Contents: VectaPlex™ Antibody Removal Reagent A VectaPlex™ Antibody Removal Reagent B FAQ pdf
- Shipping Conditions:
- Ambient
- Storage Conditions:
- RT
- Supplier:
- Vector Laboratories
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Lectins
- Can I use VectaPlex™ on frozen sections?:
- No. During development, VectaPlex™ was optimized for FFPE tissues, where the power to remove antibodies at room temperature was balanced with the need to preserve tissue morphology. For frozen tissues, VectaPlex™ will remove the tissue from the slide.
- Can VectaPlex™ remove antibodies in immunohistochemistry (IHC) applications with chromogenic staining?:
- VectaPlex™ Antibody Removal Kit is designed to be used in fluorescent workflows such as immunofluorescence or TSA. It has not been validated or confirmed for immunohistochemistry (IHC) applications. VectaPlex™ may remove substrates already deposited on tissue samples.
- I am using TrueView to quench autofluorescence, will TrueVIEW be stripped by VectaPlex™ reagents?:
- We used TrueView in our VectaPlex™ IF workflow to suppress autofluorescence. Application of VectaPlex™ removes some, but not all, of the TrueView reagent. Since some TrueView remained after VectaPlex™ application, we did not add TrueView in every cycle, but only as needed to suppress autofluorescence.
- I accidentally put my VectaPlex™ kit in the freezer. Can I still use it?:
- Yes. VectaPlex™can withstand a freeze-thaw cycle and still perform as expected, but the recommended storage is at ambient temperature (15-25 °C). Additionally, the kit will still perform properly if placed at 4 °C.
- Is there a maximum number of times you can use VectaPlex™ on one section?:
- VectaPlex™ has been tested for up to 6 cycles with no ill effects. We don't expect there to be issues with increasing the number of cycles, but we suggest testing with controls for adequate comparisons. We recommend running experiments to confirm that the use of more cycles does not impact the antigenicity or tissue morphology.