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Antibodies

RAB3D Antibody, Clone 5E9: RPE

Product Sizes
100 ug
SMC-614D-RPE-100UG
About this Product
SKU:
SMC-614D-RPE
Additional Names:
Member RAS Oncogene Family antibody, D2-2 antibody, GOV antibody, RAB16 antibody, RAD3D antibody, Ras-Related Protein Rab-3D antibody
Application:
Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
5E9
Clonality:
Monoclonal
Conjugate:
R-PE
Concentration:
1 mg/ml
Extra Details:
Rab3D is a small exocytic GTPase involved in protein transport. It is required for efficient bone resorption by osteoclasts (1) and associates with a subset of the trans-golgi network (2).
Host:
Mouse
Immunogen:
Full length recombinant Human RAB3D protein
Isotype:
IgG1
Purification:
Protein G Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Detects ~24 kDa. Does not show cross reactivity to RAB3A, RAB3B, or RAB3C.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Western Blot analysis of Human HEK293 cells overexpressing RAB3A, RAB3B, RAB3C, and RAB3D showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9 (SMC-614). Lane 1: MW ladder. Lanes 2-4: HEK293 cells overexpressing RAB3A. Lanes 5-7: HEK293 cells overexpressing RAB3B. Lanes 8-10: HEK293 cells overexpressing RAB3C. Lanes 11-13: HEK293 cells overexpressing RAB3D.. Primary Antibody: Mouse Anti-RAB3D Monoclonal Antibody (SMC-614). Courtesy of: Dario Alessi Lab, University of Dundee.
Western Blot analysis of Human, Mouse, Rat A549, T-47D cells, mouse and rat brain, kidney showing detection of RAB3D protein using Mouse Anti-RAB3D Monoclonal Antibody, Clone 5E9 (SMC-614). Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3: T-47D cells. Lane 4: mouse brain. Lane 5: rat brain. Lane 6: mouse kidney. Lane 7: rat kidney.. Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-RAB3D Monoclonal Antibody (SMC-614) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.