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Antibodies

Slo2.2 Antibody, Clone N3/26: APC

Product Sizes
100 ug
SMC-323D-APC-100UG
About this Product
SKU:
SMC-323D-APC
Additional Names:
KCNT1 Antibody, potassium channel subfamily T member 1 Antibody, bA100C15.2 Antibody, SLACK Antibody, KCa4.1KIAA1422FLJ41282 Antibody
Application:
Antibody Microarray, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
N3/26 (Formerly sold as S3-26)
Clonality:
Monoclonal
Conjugate:
APC
Concentration:
1 mg/ml
Extra Details:
Slo2.2 is a novel member of the mammalian Slo potassium channel gene family. Slo2 channels may contribute to the resting potentials of cells that control their basal level of excitability (1). They also have sensors that couple channel activity to the intracellular concentrations of Na+ and Cl- (2).
Host:
Mouse
Immunogen:
Fusion protein amino acids 1168-1237 of rat Slo2.2 (Slack)
Isotype:
IgG1
Purification:
Protein G Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Detects ~140kDa. Weak human detection. Does not cross-react with KCNT2/Slo2.1/Slick.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Slo2.2 Monoclonal Antibody, Clone N3/26 (SMC-323). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Slo2.2 Monoclonal Antibody (SMC-323) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Slo2.2 Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone N3/26 (SMC-323). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody (SMC-323) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining around nucleus and some in cytoplasm.
Western Blot analysis of Rat brain membrane lysate showing detection of Slo2.2 Potassium Channel protein using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone N3/26 (SMC-323). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody (SMC-323) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunohistochemistry analysis using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone N3/26 (SMC-323). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody (SMC-323) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Suprabasal epidermal staining. Hair follicles negative.