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Antibodies

Ubiquitin Antibody, Clone FK1: PerCP

Product Sizes
100 ug
SMC-213D-PCP-100UG
About this Product
SKU:
SMC-213D-PCP
Additional Names:
Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
95.46mM Phosphate, 2.48mM MES and 2mM EDTA
CE/IVD:
RUO
translate.label.attr.clone:
FK1
Clonality:
Monoclonal
Conjugate:
PerCP
Concentration:
1 mg/ml
Extra Details:
Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
Host:
Mouse
Immunogen:
Human Ubiquitin conjugated lysozyme
Isotype:
IgM
Purification:
Gel Filtration
Reactivities:
Wide/Non-species Specific
Shipping Conditions:
Blue Ice
Specificity:
Detects polyubiquitinated proteins and ubiquitin chains. Does not detect monoubiquitinated proteins nor free ubiquitin.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK1 (SMC-213). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-213) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.
Western Blot analysis of Poly-Ubiquitin showing detection of Ubiquitin protein using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK1 (SMC-213). Lane 1: MW ladder. Lane 2: Poly-Ubiquitin, K48-linked (2 ug). Lane 2: Poly-Ubiquitin, K63-linked (0.6 ug). Load: 5 uL. Block: 1% BSA in TBST. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-213) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:2000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.