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Antibodies

AIPL1 Antibody

Product Sizes
100 ug
600-401-X93-100UG
About this Product
SKU:
600-401-X93
Additional Names:
AIPL1|Aipl1 Antibody, LCA4, AIPL2, Aryl-hydrocarbon-interacting protein-like 1
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Aipl1 was initially identified as a protein implicated in Leber congenital amaurosis (LCA), an autosomal recessive disorder thought to be caused by the abnormal development of photoreceptors. Aipl1 is a tetratricopeptide repeat protein that is highly homologous to ARA9, a protein involved in the HSP90-mediated nuclear translocation and transactivation of the aryl hydrocarbon receptor. Aipl1 has also been found to function as part of a chaperone heterocomplex, interacting with Hsp90 and Hsp70. Aipl1 also associates with the cell cycle regulator NUB1. It is thought that Aipl1 cooperates with Hsp70 but not Hsp90 to suppress the formation of NUB1 inclusions, and these interactions are necessary in the normal photoreceptor maturation, as mutations that lead to LCA also compromise the interactions with the Hsp chaperones. At least three isoforms of Aipl1 are known to exist.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-Aipl1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 18 amino acid synthetic peptide near the internal region of the human Aipl1.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-Aipl1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with Aipl1 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry of Aipl1 antibody. Tissue: Human brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Aipl1 antibody at 2.5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Aipl1 is nuclear and occasionally cytoplasmic. Staining: Aipl1 as a precipitated red signal with hematoxylin purple nuclear counterstain.
Immunofluorescence Microscopy of Aipl1 antibody. Tissue: Human brain tissue. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Aipl1 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT Staining: Aipl1 as a red fluorescent signal.
Western Blot of Aipl1 antibody. Lane A: Rat brain tissue lysate at 1 ug/mL. Lane B: Rat brain tissue lysate at 2 ug/mL. Load: 35 ug per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 43.9 kDa, ~43 kDa for Aipl1. Other band(s): Aipl1 splice variants and isoforms.