Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

KLF4 Antibody

Product Sizes
25 ul
600-401-GU2S-25UL
About this Product
SKU:
600-401-GU2S
Additional Names:
KLF4|rabbit anti-KLF4 Antibody, KLF-4 Antibody, Krueppel-like factor 4, Epithelial zinc finger protein EZF, EZF, Gut-enriched krueppel-like factor, GKLF, KLF 4
Application:
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.05 mg/ml
Extra Details:
KLF4 belong to the Kruppel like factors/Zinc fingers C2H2-type family. This gene encodes a protein that belongs to the Kruppel family of transcription factors. KLF4 can act both as activator and as repressor. It can bind the 5-CACCC-3 core sequence, the promoter region of its own gene, and can activate its own transcription. KLF4 regulates the expression of key transcription factors during embryonic development, playing an important role in maintaining embryonic stem cells, and in preventing their differentiation. This protein is required for normal development of the barrier function of skin postnatal, maintenance of the ocular surface, function in skeletal and kidney development, and thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. KLF4 may be associated with diseases such as Venous Hemangioma, Skin Squamous Cell Carcinoma, and pediatric lymphoma. Anti-KLF4 Antibody is useful for researchers interested in Peptide hormone metabolism, Stem Cell Differentiation Pathway Research, Wnt/Notch Pathway Research, and Cancer Research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-KLF4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Term portion of human KLF4 conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against human KLF4. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human and 93% identity to mouse.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Rabbit anti-KLF4 antibody. Lane 1: MW ladder (opal pre-stained) p/n (MB-210-0500). Lane 2: U-87 WCL (p/n W09-001-GX2). Lane 3: C2C12 WCL (p/n W10-001-GL2). Lane 4: NIH/3T3 WCL (p/n W10-000-358). Lane 5: THP-1 WCL (p/n W09-001-GX1). Lane 6: J774A.1 WCL (p/n W10-001-GX3). Lane 7: PC-3 WCL (p/n W09-001-GV6). Lane 8: HT-29 WCL (p/n W09-001-BS0). Lane 9: Mouse colon (W10-000-GZ1). Lane 10: A549 WCL (p/n W09-001-372). Lane 11: MOLT-4 WCL (p/n W09-001-GK2). Lane 12: HeLa WCL (p/n W09-001-364). Lane 13: MW ladder (opal pre-stained) p/n (MB-210-0500). Load: 10 ug per lane. Primary antibody: KLF4 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n 611-103-122) at 1:70,000 for 1 hr at RT. Block: 5% BLOTTO (p/n B501-0500) 30 min at RT. Predicted/Observed size: ~55 kDa for KLF4.
Immunofluorescence Microscopy of Rabbit anti-KLF4 antibody. Tissue: PC-3. Fixation: 0.5% PFA [A,C]. 0.5% MeOH [B, D]. Antigen retrieval: not required. Primary antibody: KLF4 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Anti-RABBIT IgG DyLight™ 488 Conjugated Preadsorbed (p/n 611-741-127) at 5 ug/ml for 1 h at RT. Localization: cytoplasmic and nuclear Staining: Target as green fluorescent signal with DAPI (blue) nuclear counterstain.
Western Blot of Rabbit Anti-KLF4 Antibody. Lane 1: Opal Prestained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: NCCIT Whole Cell Lysate. Load: 30ug lysate. Primary Antibody: Rabbit Anti-KLF4 at 1ug/mL overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP Antibody (p/n 611-103-122) at 1:10,000 at RT for 1hr. Block: BLOTTO (p/n B501-0500). Predicted/Observed size: ~55 kDa for KLF4.