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Antibodies

GABA(A) Receptor alpha 1 Antibody

Product Sizes
100 ul
600-401-D43-100UL
About this Product
SKU:
600-401-D43
Additional Names:
Gabra1|Gamma-aminobutyric acid receptor subunit alpha-1, GABA(A) receptor subunit alpha-1
Application:
Immunohistochemistry, Western Blot
Buffer:
0.01 M HEPES, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six A Alphas, four B Betas and four G Gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for A Alpha- and B Beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a G Gamma-subunit is required for benzo-diazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different A Alpha-subunits of the receptor. GABA(A) receptor alpha 1 antibody is ideal for investigators involved in Neuroscience.
Formulation:
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Host:
Rabbit
Immunogen:
Anti-GABA(A) Receptor alpha 1 Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues from the N-terminal region of rat GABAA RA1 conjugated to KLH.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
GABA(A) receptor alpha 1 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification. Labeling is absent in A Alpha1-subunit knockout animals. Reactivity is expected from the following species based on 100% sequence homology: bovine, canine, human, mouse, rat, and non-human primate.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Anti-GABA(A) Receptor alpha 1 (Rabbit) Antibody. Lane 1: mouse forebrain lysates from Wild Type. Lane 2: mouse forebrain lysates from A Alpha1-knockout (A Alpha1-K/O). Load: 10 ug per lane. Primary antibody: GABAA-R antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~51kDa/~51kDa for A Alpha1-subunit of the GABAA-R. Other band(s): none.