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Antibodies

GALNT10 Antibody

Product Sizes
100 ug
600-401-BJ2-100UG
About this Product
SKU:
600-401-BJ2
Additional Names:
GALNT10|GALNT10 Antibody, GALNACT10, PPGALNACT10, PPGANTASE10, Polypeptide N-acetylgalactosaminyltransferase 10, Polypeptide GalNAc transferase 10, GalNAc-T10
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Protein glycosylation is an important biological process that is carried out by a large family of glycosyltransferases that catalyze the synthesis of oligosaccharides and glycoconjugates. Polypeptide GalNAc transferases initiate the synthesis of mucin-type oligosaccharides by transferring GalNAc from UDP-GalNAc to the hydroxyl group of either a serine or threonine residue on the polypeptide acceptor. Polypeptide galactoaminyltransferase 10 (GALNT10) belongs to the polypeptide N-acetylgalactosaminyl-transferase (pp-GalNAc-T) protein family. Following expression in insect cells, recombinant GALNT10 showed significant GalNAcT activity toward mucin-derived peptides, and it utilized both non-glycosylated and glycosylated peptide substrates. GALNT10 mRNA is highly expressed in several distinct hypothalamic, thalamic, and amygdaloid nuclei in mouse brain. At least four isoforms of GALNT10 are known to exist.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-GALNT10 antibody was prepared from whole rabbit serum produced by repeated immunizations with an 18 amino acid synthetic peptide near the C-terminus of human GALNT10.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-GALNT10 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with GALNT10 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence Microscopy of GALNT10 antibody. Tissue: Human brain cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: GALNT10 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: GALNT10 is a membrane protein. Staining: GALNT10 as red fluorescent signal.
Immunohistochemistry of GALNT10 antibody. Tissue: human brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: GALNT10 antibody at 2.5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: GALNT10 is a membrane protein. Staining: GALNT10 as precipitated brown signal with purple nuclear counterstain.
Western Blot of GALNT10 antibody. Lane 1: SK-N-SH cell lysate with GALNT10 antibody at 1 ug/mL. Lane 2: SK-N-SH cell lysate with GALNT10 antibody at 2 ug/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 69 kDa, 69 kDa for GALNT10. Other band(s): GALNT10 splice variants and isoforms.