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Antibodies

FREM1 Antibody

Product Sizes
100 ug
600-401-BH1-100UG
About this Product
SKU:
600-401-BH1
Additional Names:
FREM1|FREM1 Antibody, BNAR, MOTA, TILRR, TRIGNO2, C9orf143, C9orf145, C9orf154, FRAS1-related extracellular matrix protein 1, Protein QBRICK
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
FREM1 is a member of the FRAS1-related extracellular matrix protein family and is thought to play a role in craniofacial and renal development. FREM1 functions as an extracellular matrix protein that is essential for epidermal adhesion during embryogenesis and may also participate in epidermal differentiation. It is recognized by cells in the embryonic skin and hair follicles through different members of the integrin family. Deficiency in the Fras1/Frem genes gives rise to the bleb phenotype, which is equivalent to the human hereditary disorder Fraser syndrome.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-FREM1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide near the C-terminus of human FREM1.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-FREM1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with FREM1 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of FREM1 antibody. Lane 1: K562 cell lysate with FREM1 antibody at 0.5 ug/mL. Lane 2: K562 cell lysate with FREM1 antibody at 1 ug/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 80 kDa, 80 kDa for FREM1. Other band(s): FREM1 splice variants and isoforms.
Immunocytochemistry of FREM1 antibody. Tissue: K562 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: FREM1 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: FREM1 is secreted into the extracellular space. Staining: FREM1 as precipitated blue signal with pink nuclear counterstain.
Immunofluorescence Microscopy of FREM1 antibody. Tissue: K562 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: FREM1 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: FREM1 is secreted into the extracellular space. Staining: FREM1 as red fluorescent signal.