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Antibodies

ATF3 Antibody

Product Sizes
100 ug
600-401-493-100UG
About this Product
SKU:
600-401-493
Additional Names:
ATF3|rabbit anti-ATF3 Antibody, ATF 3, Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3, Activating transcription factor 3
Application:
ELISA, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
ATF3, or Activating Transcription Factor 3, is a member of mammalian activation TF/CREB protein family of transcription factors. ATF3 binds the cAMP response element (cre) (consensus: 5'-gtgacgt[ac][ag]-3'), a sequence present in many viral and cellular promoters. However, ATF3 represses rather than activates transcription from promoters with ATF sites stabilizing inhibitory co-factors at the promoter. Alternate splicing forms of ATF3, called ATF3 delta Zip, lack the leucine zipper domain and do not bind DNA. ATF3 delta Zip stimulates transcription, presumably by sequestering inhibitory co-factors away from the promoter. Human ATF3 (SwissProt 18847) is a 20575 Da protein composed of 181 amino acids.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATF3.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This affinity-purified antibody is directed against human ATF3 and is useful in determining its presence in various assays including ELISA and western blotting. This polyclonal antibody recognizes over-expressed ATF3 protein found in various expression systems. Reactivity is observed against human ATF3. Cross reactivity with ATF3 from other mammalian sources has not been tested.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western blot of mammalian whole cell extract transfected with HA epitope tagged human ATF3. Rockland's Affinity purified anti-ATF3 detects a band ~31 kDa corresponding to recombinant human ATF3. Immunostaining using Rockland's anti-HA epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose in 30 minutes using standard methods. After blocking with 5% goat serum and 0.5% non-fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Western blot of E.coli whole cell extract transfected with GST epitope tagged human ATF3. Rockland's Affinity purified anti-ATF3 detects a band ~48 kDa corresponding to recombinant human ATF3. Immunostaining using Rockland's anti-GST epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose using standard methods. After blocking with 5% goat serum and 0.5% non fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.