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Antibodies

Hemoglobin beta S Antibody

Product Sizes
25 ul
200-301-GS5S-25UL
About this Product
SKU:
200-301-GS5S
Additional Names:
HbBs|mouse anti-HbS antibody, mouse anti-hemoglobin antibody, Hemoglobin beta subunit sickle mutant, HBS, HBBs, HbS Antibody, Sickle Cell Disease (SCD)
Application:
ELISA, Flow Cytometry, Immunofluorescence, Lateral Flow Assay, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
23E5.H6.G6.C1.H7.F7.G9.F6
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
HbS antibodies detect the E6V mutant in the hemoglobin beta subunit. Functional adult hemoglobin (Hb) is a hetero tetramer composed of 2 alpha and 2 beta subunits (A Alpha2B Beta2). Common isoform variants of hemoglobin include HbA, HbS, HbC, HbF, and HbA2. Hemoglobin S is the predominant hemoglobin in people with sickle cell disease. The alpha chain is normal. The disease-producing mutation exists in the beta chain, giving the molecule the structure, A Alpha2B BetaS2. People who have one sickle mutant gene and one normal beta gene have sickle cell trait which is benign. Globin gene mutations affect the structure and expression levels of Hb. Sickle cell disease and the more benign sickle cell trait are observed in more than 100 million people globally. Perhaps the most significant mutation is the E6V in the beta subunit and the cause of SCD, but other relevant isoforms of Hb are observed. HbS antibody does not react to other forms of Hb. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
Anti-Hemoglobin beta S Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb B Beta-subunit conjugated to KLH.
Isotype:
IgG3, IgG3, kappa
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This protein A purified mouse monoclonal antibody reacts specifically with human HbS beta sickle isoform. Anti-HbS is purified from tissue culture supernatant by protein A purification. Blast analysis shows 100% homology to Human, Pan troglodytes, Pan paniscus, Gorilla gorilla gorilla, and Hylobates lar. This antibody does not react with the HbA, HbF, HbC, or HbA-2 isoform.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Binding mode and kinetic analysis of Mouse Anti-HbS Antibody (p/n 200-301-GS5). Using Octet R4, a series of nine (9) concentrations of Mouse Anti-HbS Antibody (3.0nM-2.2uM) were immobilized on an AR2G biosensor and exposed to 20ug/ml HbS protein. Each colored curve represents the acquired signal of its antibody concentration. The interaction features reproducible duplicate injections and fits to a 1:2 bivalent kinetic model. The association constant (ka=32x103M-1s-1) and dissociation constant (kd=8.74x10-4s-1) values are depicted in the graph. The assay was performed as per the manufacture's protocol.
Lateral Flow Results of Anti-HbA (Hemoglobin A) and Anti-HbS (Hemoglobin beta S) Antibodies. Triplicate test strips are spotted with a control, Anti-HbA antibody (p/n 200-301-GS4) 0.5uL at 250ug/mL, and Anti-HbS antibody (p/n 200-301-GN5) 0.5uL at 1mg/mL. Recombinant HbA (left group) or recombinant HbS (right group) are observed to react with the corresponding antibodies specifically, leading to blue dots. Image courtesy of team SicklED advised by Professors Xuanhong Cheng and Khanjan Mehta of Lehigh University, Bethlehem, Pennsylvania, USA.
Fitting of Kinetic data to bivalent binding site model. Steady state signals (blue filled symbols) are fitted to a bivalent binding site model (blue line). A 4-parameter non-linear regression curve fit was used for the integration of the data. The Mouse Anti-HbS Antibody (p/n 200-301-GS5) presents a KD=27nM (affinity constant) to HbS protein.
Western blot results of Mouse Anti-HbS Antibody. Lane 1: Opal Prestained molecular weight ladder - MB-210-0500. Lane 2: HbA. Lane 3: HbA2. Lane 4: HbC. Lane 5: HbF. Lane 6: HbS. Lane 7: BSA. Loaded 10ug. Blocking: BlockOut Universal buffer - MB-073 for 30 min at RT. Primary Antibody: Anti-Hemoglobin beta S at 1:1000 overnight at 4°C. Secondary Antibody: Rabbit Anti-Mouse HRP - 610-403-C46 at 1:40,000 for 30 min at RT.